PMID- 10428966
OWN - NLM
STAT- MEDLINE
DCOM- 19990916
LR  - 20161019
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 15
DP  - 1999 Aug 2
TI  - Residues phosphorylated by TFIIH are required for E2F-1 degradation during
      S-phase.
PG  - 4280-91
AB  - The transcription factor E2F-1 plays a key role in regulating cell cycle
      progression. Accordingly, E2F-1 activity is itself tightly controlled by a series
      of transcriptional and post-transcriptional events. Here we show that the E2F-1
      activation domain interacts with a kinase activity which phosphorylates two
      sites, Ser403 and Thr433, within the activation domain. We demonstrate that TFIIH
      is responsible for the E2F-1 phosphorylation observed in cell extracts and that
      endogenous E2F-1 interacts in vivo with p62, a component of TFIIH, during S
      phase. When the two phosphorylation sites in E2F-1 are mutated to alanine, the
      stability of the E2F-1 activation domain is greatly increased. These results
      suggest that TFIIH-mediated phosphorylation of E2F-1 plays a role in triggering
      E2F-1 degradation during S phase.
FAU - Vandel, L
AU  - Vandel L
AD  - Wellcome/CRC Institute, University of Cambridge, Tennis Court Road, Cambridge CB2
      1QR, UK.
FAU - Kouzarides, T
AU  - Kouzarides T
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Dp transcription factor, Drosophila)
RN  - 0 (Drosophila Proteins)
RN  - 0 (E2F Transcription Factors)
RN  - 0 (E2F1 Transcription Factor)
RN  - 0 (E2F1 protein, human)
RN  - 0 (Retinoblastoma-Binding Protein 1)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factor DP1)
RN  - 0 (Transcription Factors)
RN  - 0 (Transcription Factors, TFII)
RN  - 148710-81-0 (Transcription Factor TFIIH)
RN  - 2ZD004190S (Threonine)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.- (Protein Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Carrier Proteins
MH  - *Cell Cycle Proteins
MH  - Cell Line
MH  - *DNA-Binding Proteins
MH  - *Drosophila Proteins
MH  - E2F Transcription Factors
MH  - E2F1 Transcription Factor
MH  - HeLa Cells
MH  - Humans
MH  - Hydrolysis
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Protein Binding
MH  - Protein Kinases/metabolism
MH  - Retinoblastoma-Binding Protein 1
MH  - *S Phase
MH  - Sequence Homology, Amino Acid
MH  - Serine/metabolism
MH  - Threonine/metabolism
MH  - *Trans-Activators
MH  - Transcription Factor DP1
MH  - Transcription Factor TFIIH
MH  - Transcription Factors/chemistry/*metabolism
MH  - *Transcription Factors, TFII
MH  - Transcription, Genetic
PMC - PMC1171504
EDAT- 1999/08/03 00:00
MHDA- 1999/08/03 00:01
CRDT- 1999/08/03 00:00
PHST- 1999/08/03 00:00 [pubmed]
PHST- 1999/08/03 00:01 [medline]
PHST- 1999/08/03 00:00 [entrez]
AID - 10.1093/emboj/18.15.4280 [doi]
PST - ppublish
SO  - EMBO J. 1999 Aug 2;18(15):4280-91. doi: 10.1093/emboj/18.15.4280.