PMID- 10428842
OWN - NLM
STAT- MEDLINE
DCOM- 19990902
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 32
DP  - 1999 Aug 6
TI  - Hormone-independent transcriptional activation and coactivator binding by novel
      orphan nuclear receptor ERR3.
PG  - 22618-26
AB  - Orphan nuclear receptors share sequence homology with members of the nuclear
      receptor superfamily, but ligands are unknown or unnecessary. A novel orphan
      receptor, estrogen receptor-related protein 3 (ERR3), was identified by yeast
      two-hybrid screening, using the transcriptional coactivator glucocorticoid
      receptor interacting protein 1 (GRIP1) as bait. The putative full-length mouse
      ERR3 contains 458 amino acids and is closely related to two known orphan
      receptors ERR1 and ERR2. All the ERR family members share an almost identical
      DNA-binding domain, which has 68% amino acid identity with that of estrogen
      receptor. ERR3 bound specifically to an estrogen response element and activated
      reporter genes controlled by estrogen response elements, both in yeast and in
      mammalian cells, in the absence of any added ligand. A conserved AF-2 activation 
      domain located in the hormone-binding domain of ERR3 was primarily responsible
      for transcriptional activation. The ERR3 AF-2 domain bound GRIP1 in a
      ligand-independent manner both in vitro and in vivo, through the LXXLL motifs of 
      GRIP1, and GRIP1 functioned as a transcriptional coactivator for ERR3 in both
      yeast and mammalian cells. Expression of ERR3 in adult mouse was restricted;
      highest expression was observed in heart, kidney, and brain. In the mouse embryo 
      no expression was observed at day 7, and highest expression occurred around the
      11-15 day stages. Although ERR3 is much more closely related to ERR2 than to
      ERR1, the expression pattern for ERR3 was similar to that of ERR1 and distinct
      from that for ERR2, suggesting a unique role for ERR3 in development.
FAU - Hong, H
AU  - Hong H
AD  - Department of Pathology, University of Southern California, Los Angeles,
      California 90033, USA.
FAU - Yang, L
AU  - Yang L
FAU - Stallcup, M R
AU  - Stallcup MR
LA  - eng
SI  - GENBANK/AF117254
SI  - GENBANK/AF117255
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (ERRalpha estrogen-related receptor)
RN  - 0 (Esrrb protein, mouse)
RN  - 0 (Esrrg protein, mouse)
RN  - 0 (Estrogens)
RN  - 0 (Ligands)
RN  - 0 (Ncoa2 protein, mouse)
RN  - 0 (Nuclear Receptor Coactivator 2)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/metabolism
MH  - Estrogens
MH  - Genes, Reporter
MH  - Ligands
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nuclear Receptor Coactivator 2
MH  - Protein Binding
MH  - Receptors, Cytoplasmic and Nuclear/genetics/metabolism
MH  - Receptors, Estrogen/genetics/*metabolism
MH  - Response Elements
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
MH  - Transcription Factors/*metabolism
MH  - *Transcriptional Activation
EDAT- 1999/07/31 00:00
MHDA- 1999/07/31 00:01
CRDT- 1999/07/31 00:00
PHST- 1999/07/31 00:00 [pubmed]
PHST- 1999/07/31 00:01 [medline]
PHST- 1999/07/31 00:00 [entrez]
AID - 10.1074/jbc.274.32.22618 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Aug 6;274(32):22618-26. doi: 10.1074/jbc.274.32.22618.