PMID- 10428817
OWN - NLM
STAT- MEDLINE
DCOM- 19990902
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 32
DP  - 1999 Aug 6
TI  - TIP49b, a new RuvB-like DNA helicase, is included in a complex together with
      another RuvB-like DNA helicase, TIP49a.
PG  - 22437-44
AB  - We previously reported that TIP49a is a novel mammalian DNA helicase showing
      structural similarity with the bacterial recombination factor RuvB. In this
      study, we isolated a new TIP49a-related gene, termed TIP49b, from human and yeast
      cells. TIP49b also resembled RuvB, thus suggesting that TIP49a and TIP49b are
      included in a gene family. Like TIP49a, TIP49b was abundantly expressed in the
      testis and thymus. Enzyme assays revealed that TIP49b was an single-stranded
      DNA-stimulated ATPase and ATP-dependent DNA helicase. Most of the enzymatic
      properties of TIP49b were the same as those of TIP49a, whereas the polarity of
      TIP49b DNA helicase activity (5' to 3') was the opposite to that of TIP49a.
      TIP49b and TIP49a bound to each other and were included in the same complex of
      approximately 700 kDa in a cell. We found that TIP49b was an essential gene for
      the growth of Saccharomyces cerevisiae, as is the TIP49a gene, suggesting that
      TIP49b does not complement the TIP49a function and vice versa. From these
      observations, we suggest that TIP49b plays an essential role in the cellular
      processes involved in DNA metabolism.
FAU - Kanemaki, M
AU  - Kanemaki M
AD  - Department of Biology, Faculty of Science, Chiba University, CREST Japan Science 
      and Technology Corp., 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
FAU - Kurokawa, Y
AU  - Kurokawa Y
FAU - Matsu-ura, T
AU  - Matsu-ura T
FAU - Makino, Y
AU  - Makino Y
FAU - Masani, A
AU  - Masani A
FAU - Okazaki, K
AU  - Okazaki K
FAU - Morishita, T
AU  - Morishita T
FAU - Tamura, T A
AU  - Tamura TA
LA  - eng
SI  - GENBANK/AB024301
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Bacterial Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RuvB protein, Bacteria)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.4.- (ATPases Associated with Diverse Cellular Activities)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 3.6.4.12 (RUVBL1 protein, human)
RN  - EC 3.6.4.12 (RUVBL2 protein, human)
SB  - IM
MH  - ATPases Associated with Diverse Cellular Activities
MH  - Adenosine Triphosphatases/metabolism
MH  - Amino Acid Sequence
MH  - Bacterial Proteins/genetics
MH  - Base Sequence
MH  - Carrier Proteins/genetics/*isolation & purification/metabolism
MH  - Cloning, Molecular
MH  - DNA Helicases/genetics/*isolation & purification/metabolism
MH  - DNA, Complementary/genetics
MH  - Eukaryotic Cells/enzymology
MH  - Genes, Essential
MH  - Genes, Fungal
MH  - Humans
MH  - Macromolecular Substances
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/genetics/isolation & purification/metabolism
MH  - Protein Binding
MH  - Saccharomyces cerevisiae/genetics
MH  - Tissue Distribution
EDAT- 1999/07/31 00:00
MHDA- 1999/07/31 00:01
CRDT- 1999/07/31 00:00
PHST- 1999/07/31 00:00 [pubmed]
PHST- 1999/07/31 00:01 [medline]
PHST- 1999/07/31 00:00 [entrez]
AID - 10.1074/jbc.274.32.22437 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Aug 6;274(32):22437-44. doi: 10.1074/jbc.274.32.22437.