PMID- 10428810
OWN - NLM
STAT- MEDLINE
DCOM- 19990902
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 32
DP  - 1999 Aug 6
TI  - Kinase activity and phosphorylation of the largest subunit of TFIIF transcription
      factor.
PG  - 22387-92
AB  - The largest subunit of the human basal transcription factor TFIIFalpha (also
      called RAP74) was reported previously to be the target of some
      phospho/dephosphorylation process. We show that TFIIFalpha possesses a
      serine/threonine kinase activity, allowing an autophosphorylation of the two
      residues at position serine 385 and threonine 389. Mutation analysis strongly
      suggests that autophosphorylation of both sites regulates the transcription
      elongation process. Moreover we also evidence three additional phosphorylation
      sites located at positions 207-230, 271-283, and 335-344. These sites are
      phosphorylated by casein kinase II-like kinases and TAF(II)250, a component of
      TFIID.
FAU - Rossignol, M
AU  - Rossignol M
AD  - Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS/INSERM/ULP, 
      B. P.163, 67404 Illkirch Cedex, Communaute Urbaine de Strasbourg, France.
FAU - Keriel, A
AU  - Keriel A
FAU - Staub, A
AU  - Staub A
FAU - Egly, J M
AU  - Egly JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (TATA-Binding Protein Associated Factors)
RN  - 0 (Transcription Factor TFIID)
RN  - 0 (Transcription Factors)
RN  - 0 (Transcription Factors, TFII)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.7.11.1 (Casein Kinase II)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (TATA-binding protein associated factor 250 kDa)
RN  - EC 3.6.4.12 (transcription factor TFIIF)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Casein Kinase II
MH  - DNA Mutational Analysis
MH  - DNA-Binding Proteins/metabolism
MH  - Histone Acetyltransferases
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/metabolism
MH  - Phosphorylation
MH  - Protein-Serine-Threonine Kinases/genetics/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Spodoptera/genetics
MH  - Substrate Specificity
MH  - *TATA-Binding Protein Associated Factors
MH  - Transcription Factor TFIID
MH  - Transcription Factors/genetics/*metabolism
MH  - Transcription Factors, TFII/metabolism
MH  - *Transcription, Genetic
EDAT- 1999/07/31 00:00
MHDA- 1999/07/31 00:01
CRDT- 1999/07/31 00:00
PHST- 1999/07/31 00:00 [pubmed]
PHST- 1999/07/31 00:01 [medline]
PHST- 1999/07/31 00:00 [entrez]
AID - 10.1074/jbc.274.32.22387 [doi]
AID - S0021-9258(18)81553-8 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Aug 6;274(32):22387-92. doi: 10.1074/jbc.274.32.22387.