PMID- 10428808
OWN - NLM
STAT- MEDLINE
DCOM- 19990902
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 32
DP  - 1999 Aug 6
TI  - Isolation and characterization of ARA160 as the first androgen receptor
      N-terminal-associated coactivator in human prostate cells.
PG  - 22373-9
AB  - The androgen receptor (AR) is a member of the steroid receptor superfamily that
      may require coactivators for proper or maximal transactivation. Using a purified 
      AR N-terminal peptide as a probe to screen the human testis expression library,
      we identified an androgen-enhanced AR N-terminal-associated protein ARA160, which
      consists of 1,093 amino acids with an apparent molecular mass of 160 kDa.
      Sequence comparison in GenBank(TM) reveals that ARA160 shares an identical
      sequence with a HIV-1 TATA element modulatory factor, TMF. The far-Western
      blotting and co-immunoprecipitation assays demonstrate that the AR can interact
      directly with ARA160/TMF. Affinity gel pull-down and mammalian two-hybrid assays 
      further suggest androgen can enhance significantly the interaction between AR and
      ARA160. Transient transfection assays demonstrated that ARA160 might function as 
      a coactivator for AR-mediated transactivation in human prostate cancer PC-3
      cells. Our data further suggest that this AR N-terminal coactivator can function 
      cooperatively with AR C-terminal coactivator, ARA70, in PC-3 cells. Together, our
      data demonstrate that ARA160 might represent the first identified
      androgen-enhanced N-terminal coactivator for the AR.
FAU - Hsiao, P W
AU  - Hsiao PW
AD  - Departments of Pathology, Urology, and Radiation Oncology, George Whipple
      Laboratory for Cancer Research, University of Rochester, Rochester, New York
      14642, USA.
FAU - Chang, C
AU  - Chang C
LA  - eng
GR  - CA55639/CA/NCI NIH HHS/United States
GR  - CA68518/CA/NCI NIH HHS/United States
GR  - CA71570/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Androgens)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Ligands)
RN  - 0 (NCOA4 protein, human)
RN  - 0 (Nuclear Receptor Coactivators)
RN  - 0 (Oncogene Proteins)
RN  - 0 (Receptors, Androgen)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 149954-67-6 (TMF1 protein, human)
RN  - EC 3.4.21.77 (Prostate-Specific Antigen)
SB  - IM
SB  - X
MH  - Amino Acid Sequence
MH  - Androgens/*metabolism
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/genetics/isolation & purification/*metabolism
MH  - Humans
MH  - Ligands
MH  - Male
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Molecular Sequence Data
MH  - Nuclear Receptor Coactivators
MH  - *Oncogene Proteins
MH  - Promoter Regions, Genetic
MH  - Prostate/chemistry/*metabolism
MH  - Prostate-Specific Antigen/genetics
MH  - Protein Binding
MH  - Receptors, Androgen/*metabolism
MH  - Receptors, Estrogen/metabolism
MH  - Receptors, Glucocorticoid/metabolism
MH  - Response Elements
MH  - Sequence Analysis, DNA
MH  - Terminal Repeat Sequences
MH  - Trans-Activators/metabolism
MH  - Transcription Factors/genetics/isolation & purification/*metabolism
MH  - Transcriptional Activation
EDAT- 1999/07/31 00:00
MHDA- 1999/07/31 00:01
CRDT- 1999/07/31 00:00
PHST- 1999/07/31 00:00 [pubmed]
PHST- 1999/07/31 00:01 [medline]
PHST- 1999/07/31 00:00 [entrez]
AID - 10.1074/jbc.274.32.22373 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Aug 6;274(32):22373-9. doi: 10.1074/jbc.274.32.22373.