PMID- 10428477
OWN - NLM
STAT- MEDLINE
DCOM- 19990816
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 455
IP  - 1-2
DP  - 1999 Jul 16
TI  - Casein kinase II is responsible for phosphorylation of NF-L at Ser-473.
PG  - 83-6
AB  - Ser-473 is solely phosphorylated in vivo in the tail region of neurofilament L
      (NF-L). With peptides including the native phosphorylation site, it was not
      possible to locate responsible kinases. We therefore adopted full-length
      dephosphorylated NF-L as the substrate, and employed MALDI/TOF (matrix-assisted
      laser desorption and ionization/time of flight) mass spectrometry and a
      site-specific phosphorylation-dependent antibody recognizing Ser-473
      phosphorylation. The antibody showed that casein kinase I (CK I) as well as
      casein kinase II (CK II) phosphorylated Ser-473 in vitro, while neither GSK-3beta
      nor calcium/calmodulin-dependent protein kinase II did so. However, the mass
      spectra of the tail fragments of the phosphorylated NF-L indicated that CK II was
      the kinase mediating Ser-473 phosphorylation in vitro as opposed to CK I, because
      CK I phosphorylated another site as well as Ser-473 in vitro. The antibody also
      demonstrated that NF-L phosphorylated at Ser-473 was abundant in the neuronal
      perikarya of the rat cortex, indicating that phosphorylation of Ser-473 may take 
      place there. This result may support the suggestion that CK II is the kinase
      responsible for Ser-473 phosphorylation. Despite many reports showing that CK I
      mediates phosphorylation of neurofilaments, CK II may phosphorylate NF-L in vivo.
FAU - Nakamura, Y
AU  - Nakamura Y
AD  - Department of Clinical Neuroscience, Psychiatry, Osaka University Graduate School
      of Medicine, Suita-shi, Japan. nakamura@psy.med.osaka-u.ac.jp
FAU - Hashimoto, R
AU  - Hashimoto R
FAU - Kashiwagi, Y
AU  - Kashiwagi Y
FAU - Wada, Y
AU  - Wada Y
FAU - Sakoda, S
AU  - Sakoda S
FAU - Miyamae, Y
AU  - Miyamae Y
FAU - Kudo, T
AU  - Kudo T
FAU - Takeda, M
AU  - Takeda M
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Neurofilament Proteins)
RN  - 0 (neurofilament protein L)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.11.1 (Casein Kinase II)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibody Specificity
MH  - Casein Kinase II
MH  - Cattle
MH  - Immunohistochemistry
MH  - Neurofilament Proteins/chemistry/immunology/*metabolism
MH  - Phosphorylation
MH  - Protein-Serine-Threonine Kinases/*metabolism
MH  - Rats
MH  - Serine/*metabolism
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 1999/07/31 00:00
MHDA- 1999/07/31 00:01
CRDT- 1999/07/31 00:00
PHST- 1999/07/31 00:00 [pubmed]
PHST- 1999/07/31 00:01 [medline]
PHST- 1999/07/31 00:00 [entrez]
AID - S0014-5793(99)00832-7 [pii]
AID - 10.1016/s0014-5793(99)00832-7 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Jul 16;455(1-2):83-6. doi: 10.1016/s0014-5793(99)00832-7.