PMID- 10428027
OWN - NLM
STAT- MEDLINE
DCOM- 19990813
LR  - 20220414
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 98
IP  - 2
DP  - 1999 Jul 23
TI  - Targeted deficiency or cytosolic truncation of the VE-cadherin gene in mice
      impairs VEGF-mediated endothelial survival and angiogenesis.
PG  - 147-57
AB  - Vascular endothelial cadherin, VE-cadherin, mediates adhesion between endothelial
      cells and may affect vascular morphogenesis via intracellular signaling, but the 
      nature of these signals remains unknown. Here, targeted inactivation (VEC-/-) or 
      truncation of the beta-catenin-binding cytosolic domain (VECdeltaC/deltaC) of the
      VE-cadherin gene was found not to affect assembly of endothelial cells in
      vascular plexi, but to impair their subsequent remodeling and maturation, causing
      lethality at 9.5 days of gestation. Deficiency or truncation of VE-cadherin
      induced endothelial apoptosis and abolished transmission of the endothelial
      survival signal by VEGF-A to Akt kinase and Bcl2 via reduced complex formation
      with VEGF receptor-2, beta-catenin, and phosphoinositide 3 (PI3)-kinase. Thus,
      VE-cadherin/ beta-catenin signaling controls endothelial survival.
FAU - Carmeliet, P
AU  - Carmeliet P
AD  - Center for Transgene Technology and Gene Therapy, Flanders Interuniversity
      Institute for Biotechnology, Leuven, Belgium. peter.carmelilet@med.kuleuven.ac.be
FAU - Lampugnani, M G
AU  - Lampugnani MG
FAU - Moons, L
AU  - Moons L
FAU - Breviario, F
AU  - Breviario F
FAU - Compernolle, V
AU  - Compernolle V
FAU - Bono, F
AU  - Bono F
FAU - Balconi, G
AU  - Balconi G
FAU - Spagnuolo, R
AU  - Spagnuolo R
FAU - Oosthuyse, B
AU  - Oosthuyse B
FAU - Dewerchin, M
AU  - Dewerchin M
FAU - Zanetti, A
AU  - Zanetti A
FAU - Angellilo, A
AU  - Angellilo A
FAU - Mattot, V
AU  - Mattot V
FAU - Nuyens, D
AU  - Nuyens D
FAU - Lutgens, E
AU  - Lutgens E
FAU - Clotman, F
AU  - Clotman F
FAU - de Ruiter, M C
AU  - de Ruiter MC
FAU - Gittenberger-de Groot, A
AU  - Gittenberger-de Groot A
FAU - Poelmann, R
AU  - Poelmann R
FAU - Lupu, F
AU  - Lupu F
FAU - Herbert, J M
AU  - Herbert JM
FAU - Collen, D
AU  - Collen D
FAU - Dejana, E
AU  - Dejana E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Antigens, CD)
RN  - 0 (CTNNB1 protein, mouse)
RN  - 0 (Cadherins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Lymphokines)
RN  - 0 (Receptors, Growth Factor)
RN  - 0 (Trans-Activators)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
RN  - 0 (beta Catenin)
RN  - 0 (cadherin 5)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptors, Vascular Endothelial Growth Factor)
SB  - IM
MH  - Animals
MH  - Antigens, CD
MH  - Apoptosis/physiology
MH  - Cadherins/*genetics
MH  - Cell Survival/physiology
MH  - Cytoskeletal Proteins/physiology
MH  - Cytosol/chemistry/physiology
MH  - DNA Primers
MH  - Endothelial Growth Factors/*physiology
MH  - Endothelium, Vascular/chemistry/*cytology/ultrastructure
MH  - Fetus/cytology
MH  - Gene Expression Regulation, Developmental
MH  - Hematopoiesis/physiology
MH  - In Situ Nick-End Labeling
MH  - Intercellular Junctions/physiology
MH  - Lymphokines/*physiology
MH  - Mice
MH  - Mice, Transgenic
MH  - Microscopy, Electron
MH  - Mutagenesis, Site-Directed
MH  - Neovascularization, Physiologic/*physiology
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Receptor Protein-Tyrosine Kinases/physiology
MH  - Receptors, Growth Factor/physiology
MH  - Receptors, Vascular Endothelial Growth Factor
MH  - Signal Transduction/physiology
MH  - *Trans-Activators
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
MH  - beta Catenin
EDAT- 1999/07/31 00:00
MHDA- 1999/07/31 00:01
CRDT- 1999/07/31 00:00
PHST- 1999/07/31 00:00 [pubmed]
PHST- 1999/07/31 00:01 [medline]
PHST- 1999/07/31 00:00 [entrez]
AID - S0092-8674(00)81010-7 [pii]
AID - 10.1016/s0092-8674(00)81010-7 [doi]
PST - ppublish
SO  - Cell. 1999 Jul 23;98(2):147-57. doi: 10.1016/s0092-8674(00)81010-7.