PMID- 10426995
OWN - NLM
STAT- MEDLINE
DCOM- 19990816
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 285
IP  - 5428
DP  - 1999 Jul 30
TI  - Host defense mechanisms triggered by microbial lipoproteins through toll-like
      receptors.
PG  - 732-6
AB  - The generation of cell-mediated immunity against many infectious pathogens
      involves the production of interleukin-12 (IL-12), a key signal of the innate
      immune system. Yet, for many pathogens, the molecules that induce IL-12
      production by macrophages and the mechanisms by which they do so remain
      undefined. Here it is shown that microbial lipoproteins are potent stimulators of
      IL-12 production by human macrophages, and that induction is mediated by
      Toll-like receptors (TLRs). Several lipoproteins stimulated TLR-dependent
      transcription of inducible nitric oxide synthase and the production of nitric
      oxide, a powerful microbicidal pathway. Activation of TLRs by microbial
      lipoproteins may initiate innate defense mechanisms against infectious pathogens.
FAU - Brightbill, H D
AU  - Brightbill HD
AD  - Department of Microbiology and Immunology, Howard Hughes Medical Institute,
      University of California Los Angeles School of Medicine, Los Anges, CA 90095,
      USA.
FAU - Libraty, D H
AU  - Libraty DH
FAU - Krutzik, S R
AU  - Krutzik SR
FAU - Yang, R B
AU  - Yang RB
FAU - Belisle, J T
AU  - Belisle JT
FAU - Bleharski, J R
AU  - Bleharski JR
FAU - Maitland, M
AU  - Maitland M
FAU - Norgard, M V
AU  - Norgard MV
FAU - Plevy, S E
AU  - Plevy SE
FAU - Smale, S T
AU  - Smale ST
FAU - Brennan, P J
AU  - Brennan PJ
FAU - Bloom, B R
AU  - Bloom BR
FAU - Godowski, P J
AU  - Godowski PJ
FAU - Modlin, R L
AU  - Modlin RL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Ag38 antigen, Mycobacterium tuberculosis)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Lipoproteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Toll-Like Receptors)
RN  - 187348-17-0 (Interleukin-12)
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, mouse)
SB  - IM
MH  - Animals
MH  - Antigens, Bacterial/chemistry/*immunology/metabolism
MH  - Cell Line
MH  - *Drosophila Proteins
MH  - Gene Expression Regulation
MH  - Humans
MH  - Interleukin-12/*biosynthesis/genetics
MH  - Lipopolysaccharides/immunology
MH  - Lipoproteins/chemistry/*immunology/metabolism
MH  - Macrophages/*immunology/metabolism
MH  - Membrane Glycoproteins/*metabolism
MH  - Mice
MH  - Monocytes/*immunology/metabolism
MH  - Mycobacterium tuberculosis/*immunology
MH  - NF-kappa B/biosynthesis
MH  - Nitric Oxide Synthase/genetics
MH  - Nitric Oxide Synthase Type II
MH  - Promoter Regions, Genetic
MH  - Receptors, Cell Surface/*metabolism
MH  - Signal Transduction
MH  - Toll-Like Receptors
MH  - Transcription, Genetic
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1999/07/31 00:00
MHDA- 1999/07/31 00:01
CRDT- 1999/07/31 00:00
PHST- 1999/07/31 00:00 [pubmed]
PHST- 1999/07/31 00:01 [medline]
PHST- 1999/07/31 00:00 [entrez]
AID - 7722 [pii]
AID - 10.1126/science.285.5428.732 [doi]
PST - ppublish
SO  - Science. 1999 Jul 30;285(5428):732-6. doi: 10.1126/science.285.5428.732.