PMID- 10425208
OWN - NLM
STAT- MEDLINE
DCOM- 19990908
LR  - 20161124
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 261
IP  - 2
DP  - 1999 Aug 2
TI  - The p38MAPK inhibitor SB203580 alleviates ultraviolet-induced phosphorylation at 
      serine 389 but not serine 15 and activation of p53.
PG  - 464-71
AB  - Phosphorylation of p53 at serine 389 has been shown to be responsive uniquely to 
      UV but not gamma irradiation. This report describes identification of the
      UV-responsive p38MAPK protein as a serine 389 kinase. The immunoprecipitated
      p38MAPK from UV-irradiated murine embryonic testicular carcinoma F9 cells
      phosphorylated the serine 392 residue but not serine 15 of the human p53 protein 
      in vitro and this phosphorylation was inhibited by a p38MAPK-specific chemical
      inhibitor SB203580. The inhibitor also remarkably alleviated the UV-caused
      induction and serine 389 but not serine 15 phosphorylation of the murine p53
      protein in vivo. Subsequently, this compound suppressed transcriptional activity 
      of p53 and partially retarded UV-induced apoptosis. Moreover, p53 bound to p38 as
      revealed by immunoprecipitation with anti-p53 antibodies from UV-treated F9
      cells. Thus, these results suggest that UV-stimulated p53 phosphorylation at
      serine 389 is mediated by the stress-responsive p38MAPK.
CI  - Copyright 1999 Academic Press.
FAU - Keller, D
AU  - Keller D
AD  - Department of Biochemistry and Molecular Biology, Department of Cell and
      Development Biology, Oregon Health Sciences University, 3181 SW Sam Jackson Park 
      Road, Portland, Oregon, 97201, USA.
FAU - Zeng, X
AU  - Zeng X
FAU - Li, X
AU  - Li X
FAU - Kapoor, M
AU  - Kapoor M
FAU - Iordanov, M S
AU  - Iordanov MS
FAU - Taya, Y
AU  - Taya Y
FAU - Lozano, G
AU  - Lozano G
FAU - Magun, B
AU  - Magun B
FAU - Lu, H
AU  - Lu H
LA  - eng
GR  - CA-39360/CA/NCI NIH HHS/United States
GR  - ES-08456/ES/NIEHS NIH HHS/United States
GR  - R01 CA 79721/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Imidazoles)
RN  - 0 (Pyridines)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - OU13V1EYWQ (SB 203580)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects/radiation effects
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*antagonists & inhibitors/metabolism
MH  - Enzyme Inhibitors/*pharmacology
MH  - Humans
MH  - Imidazoles/*pharmacology
MH  - In Vitro Techniques
MH  - Male
MH  - Mice
MH  - *Mitogen-Activated Protein Kinases
MH  - Phosphorylation
MH  - Pyridines/*pharmacology
MH  - Serine/chemistry
MH  - Testicular Neoplasms/enzymology
MH  - Transcription, Genetic/drug effects/radiation effects
MH  - Tumor Cells, Cultured
MH  - Tumor Suppressor Protein p53/chemistry/*metabolism/*radiation effects
MH  - Ultraviolet Rays
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 1999/07/30 00:00
MHDA- 1999/07/30 00:01
CRDT- 1999/07/30 00:00
PHST- 1999/07/30 00:00 [pubmed]
PHST- 1999/07/30 00:01 [medline]
PHST- 1999/07/30 00:00 [entrez]
AID - 10.1006/bbrc.1999.1023 [doi]
AID - S0006-291X(99)91023-9 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 2;261(2):464-71. doi:
      10.1006/bbrc.1999.1023.