PMID- 10425196
OWN - NLM
STAT- MEDLINE
DCOM- 19990908
LR  - 20071115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 261
IP  - 2
DP  - 1999 Aug 2
TI  - Molecular cloning and expression analysis of a mouse phospholipase C-delta1.
PG  - 393-9
AB  - We describe here the molecular cloning and expression analysis of mouse
      PLC-delta1 (mPLC-delta1), a key enzyme in cell signal transduction. A mouse brain
      cDNA library was screened in order to isolate the mPLC-delta1 cDNA. The
      mPLC-delta1 cDNA was 2660 bp in length. The predicted open reading frame encodes 
      a protein of 756 amino acids with an estimated molecular mass of 85 kDa. The
      deduced amino acid sequence exhibits 96.9% and 92.7% identity with the sequence
      of rat and human PLC-delta1, respectively. The mPLC-delta1 mRNA was highly
      expressed in brain, heart, lung, and testis. We found that transcripts of
      mPLC-delta1 are present in almost all regions of mouse brain examined, implying
      that the enzyme may play a role in some fundamental cellular process in brain. In
      male reproductive tract, mPLC-delta1 mRNA was widely expressed in the epididymis 
      as well as in the testis. In situ hybridization studies indicate that
      distribution of mPLC-delta1 mRNA in mouse testis is discrete and unique. The
      expression of mPLC-delta1 mRNA was defined in the periphery of each seminiferous 
      tubule, especially in spermatogonia, which might imply that mPLC-delta1 plays a
      role in proliferation of spermatogonia. To the best our knowledge, this is the
      first report to demonstrate the high expression of mPLC-delta1 mRNA in
      spermatogonia of testis. Taken together, these results suggest that mPLC-delta1
      may carry out fundamental roles in almost all of mouse tissues, especially in
      brain and specific roles in testis.
CI  - Copyright 1999 Academic Press.
FAU - Lee, W K
AU  - Lee WK
AD  - College of Medicine, Catholic University of Korea, 505 Banpo-dong, Socho-ku,
      Seoul, 137-701, Korea.
FAU - Kim, J K
AU  - Kim JK
FAU - Seo, M S
AU  - Seo MS
FAU - Cha, J H
AU  - Cha JH
FAU - Lee, K J
AU  - Lee KJ
FAU - Rha, H K
AU  - Rha HK
FAU - Min, D S
AU  - Min DS
FAU - Jo, Y H
AU  - Jo YH
FAU - Lee, K H
AU  - Lee KH
LA  - eng
SI  - GENBANK/AF133125
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.11 (PLCD1 protein, human)
RN  - EC 3.1.4.11 (Phospholipase C delta)
RN  - EC 3.1.4.11 (Plcd1 protein, mouse)
RN  - EC 3.1.4.11 (Plcd1 protein, rat)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/enzymology
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics
MH  - Gene Expression
MH  - Genitalia, Male/enzymology
MH  - Humans
MH  - In Situ Hybridization
MH  - Isoenzymes/*genetics
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phospholipase C delta
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Tissue Distribution
MH  - Type C Phospholipases/*genetics
EDAT- 1999/07/30 00:00
MHDA- 1999/07/30 00:01
CRDT- 1999/07/30 00:00
PHST- 1999/07/30 00:00 [pubmed]
PHST- 1999/07/30 00:01 [medline]
PHST- 1999/07/30 00:00 [entrez]
AID - 10.1006/bbrc.1999.1035 [doi]
AID - S0006-291X(99)91035-5 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 2;261(2):393-9. doi: 10.1006/bbrc.1999.1035.