PMID- 10425179
OWN - NLM
STAT- MEDLINE
DCOM- 19990908
LR  - 20171116
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 261
IP  - 2
DP  - 1999 Aug 2
TI  - CD31 (PECAM-1) exists as a dimer and is heavily N-glycosylated.
PG  - 283-91
AB  - CD31 (PECAM-1) is a highly abundant cell surface glycoprotein expressed on
      hemopoietic and endothelial cells where it functions as a homophilic adhesion and
      signaling receptor. Since dimerization and appropriate glycosylation are
      important features in the regulation of cell surface interactions and signal
      transduction, we studied the pattern of glycosylation as well as the ability of
      CD31 to undergo dimerization, both in solution and when expressed on cell
      membranes. CD31 is heavily glycosylated, with an approximate carbohydrate content
      of 21%. Nineteen neutral and thirteen sialylated glycans were identified.
      Ultracentrifugation analysis showed that soluble recombinant CD31 exists in
      equilibrium between a monomer and a dimer with an approximate dissociation
      constant of 12.5 microM. Chemical cross-linking studies of both soluble and
      membrane-expressed CD31 confirmed that CD31 exists as a dimer. These studies
      suggest that, like E-cadherin, PECAM-dimerization is likely to play a role in
      CD31 adhesion and signaling.
CI  - Copyright 1999 Academic Press.
FAU - Newton, J P
AU  - Newton JP
AD  - Cell Adhesion Laboratory, Institute of Molecular Medicine, Headington, OX3 9DU,
      United Kingdom.
FAU - Hunter, A P
AU  - Hunter AP
FAU - Simmons, D L
AU  - Simmons DL
FAU - Buckley, C D
AU  - Buckley CD
FAU - Harvey, D J
AU  - Harvey DJ
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (DNA Primers)
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - 0 (Polysaccharides)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Solutions)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Carbohydrate Sequence
MH  - Cell Adhesion
MH  - Cell Membrane/metabolism
MH  - Cross-Linking Reagents
MH  - DNA Primers/genetics
MH  - Dimerization
MH  - Glycosylation
MH  - Humans
MH  - In Vitro Techniques
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Platelet Endothelial Cell Adhesion Molecule-1/*chemistry/genetics/metabolism
MH  - Polysaccharides/chemistry
MH  - Protein Conformation
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Signal Transduction
MH  - Solutions
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 1999/07/30 00:00
MHDA- 1999/07/30 00:01
CRDT- 1999/07/30 00:00
PHST- 1999/07/30 00:00 [pubmed]
PHST- 1999/07/30 00:01 [medline]
PHST- 1999/07/30 00:00 [entrez]
AID - 10.1006/bbrc.1999.1018 [doi]
AID - S0006-291X(99)91018-5 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 2;261(2):283-91. doi:
      10.1006/bbrc.1999.1018.