PMID- 10424882
OWN - NLM
STAT- MEDLINE
DCOM- 19990908
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 47
IP  - 8
DP  - 1999 Aug
TI  - Immunohistochemical detection of alpha1E voltage-gated Ca(2+) channel isoforms in
      cerebellum, INS-1 cells, and neuroendocrine cells of the digestive system.
PG  - 981-94
AB  - Polyclonal antibodies were raised against a common and a specific epitope present
      only in longer alpha1E isoforms of voltage-gated Ca(2+) channels, yielding an
      "anti-E-com" and an "anti-E-spec" serum, respectively. The specificity of both
      sera was established by immunocytochemistry and immunoblotting using stably
      transfected HEK-293 cells or membrane proteins derived from them. Cells from the 
      insulinoma cell line INS-1, tissue sections from cerebellum, and representative
      regions of gastrointestinal tract were stained immunocytochemically. INS-1 cells 
      expressed an alpha1E splice variant with a longer carboxy terminus, the so-called
      alpha1Ee isoform. Similarily, in rat cerebellum, which was used as a reference
      system, the anti-E-spec serum stained somata and dendrites of Purkinje cells.
      Only faint staining was seen throughout the cerebellar granule cell layer. After 
      prolonged incubation times, neurons of the molecular layer were stained by
      anti-E-com, suggesting that a shorter alpha1E isoform is expressed at a lower
      protein density. In human gastrointestinal tract, endocrine cells of the antral
      mucosa (stomach), small and large intestine, and islets of Langerhans were
      stained by the anti-E-spec serum. In addition, staining by the anti-E-spec serum 
      was observed in Paneth cells and in the smooth muscle cell layer of the lamina
      muscularis mucosae. We conclude that the longer alpha1Ee isoform is expressed in 
      neuroendocrine cells of the digestive system and that, in pancreas, alpha1Ee
      expression is restricted to the neuroendocrine part, the islets of Langerhans.
      alpha1E therefore appears to be a common voltage-gated Ca(2+) channel linked to
      neuroendocrine and related systems of the body.
FAU - Grabsch, H
AU  - Grabsch H
AD  - Institutes of Neurophysiology, University of Cologne, Cologne, Germany.
FAU - Pereverzev, A
AU  - Pereverzev A
FAU - Weiergraber, M
AU  - Weiergraber M
FAU - Schramm, M
AU  - Schramm M
FAU - Henry, M
AU  - Henry M
FAU - Vajna, R
AU  - Vajna R
FAU - Beattie, R E
AU  - Beattie RE
FAU - Volsen, S G
AU  - Volsen SG
FAU - Klockner, U
AU  - Klockner U
FAU - Hescheler, J
AU  - Hescheler J
FAU - Schneider, T
AU  - Schneider T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the
      Histochemistry Society
JID - 9815334
RN  - 0 (Calcium Channels)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Animals
MH  - Antibody Specificity
MH  - Calcium Channels/immunology/*metabolism
MH  - Cerebellum/*metabolism
MH  - Digestive System/*metabolism
MH  - Humans
MH  - Insulinoma/metabolism
MH  - Islets of Langerhans/*metabolism
MH  - Protein Isoforms/immunology/metabolism
MH  - Rats
MH  - Tumor Cells, Cultured
EDAT- 1999/07/29 00:00
MHDA- 1999/07/29 00:01
CRDT- 1999/07/29 00:00
PHST- 1999/07/29 00:00 [pubmed]
PHST- 1999/07/29 00:01 [medline]
PHST- 1999/07/29 00:00 [entrez]
AID - 10.1177/002215549904700802 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1999 Aug;47(8):981-94. doi: 10.1177/002215549904700802.