PMID- 10424878
OWN - NLM
STAT- MEDLINE
DCOM- 20041014
LR  - 20190905
IS  - 0340-2061 (Print)
IS  - 0340-2061 (Linking)
VI  - 200
IP  - 2
DP  - 1999 Aug
TI  - Immunohistochemistry of myoepithelial cells during development of the rat
      salivary glands.
PG  - 215-28
AB  - Using a battery of monoclonal antibodies specific for rat proteins,
      immunohistochemistry was carried out on the developing myoepithelial cells (MECs)
      of the rat major salivary glands. The proteins examined were alpha-smooth muscle 
      actin (alphaSMA), h1-calponin (calponin), keratin 14 (K14), beta subunit of S-100
      protein (S-100beta), vimentin and glial fibrillary acidic protein (GFAP). The
      MECs exhibited immunoreactivity for alphaSMA, calponin and K14, but not that for 
      S-100beta, vimentin and GFAP. Immunoreactivity for alphaSMA appeared in the MECs 
      from the time when the microfilaments were initially deposited in these cells,
      i.e., at 20 days in utero in the sublingual and submandibular glands and at birth
      in the parotid gland. Calponin immunoreactivity was seen 1 day earlier than
      alphaSMA. The appearance was almost at the same time as the onset of the MEC
      differentiation in each gland. A small number of the MECs expressed weak K14
      immunoreactivity from the time when the acinus-intercalated duct structure was
      established, i.e., at 21 days in utero in the sublingual gland, at 5 days after
      birth in the perotid gland and after 5 weeks post-natally in the submandibular
      gland. In addition, K14 immunoreactivity was observed in the basal cells of the
      striated and excretory ducts. The first appearance of K14 in these cells again
      coincided with the emergence of the duct system in each gland, i.e., at 20 days
      in utero in the sublingual gland, at 21 days in utero in the submandibular gland 
      and at 3 days after birth in the parotid gland. Finally, the MECs in all the
      glands were found to redistribute as the acini matured. As the acini grew rapidly
      during the weaning period in the parotid and the sublingual glands, the MECs
      ceased to surround the acini. Thereafter, they disappeared from the acini in the 
      parotid gland, whereas they reappeared in the sublingual gland. In the
      submandibular gland, the MECs were confined to the terminal tubules until 4 weeks
      after birth. Thereafter, the acini were established and invested by the MECs. In 
      conclusion, immunohistochemistry of calponin and alphaSMA is a useful tool for
      identification of the MEC during its earliest differentiation, which has hitherto
      been possible only electron microscopically. In addition, it is suggested that
      the MEC is heterogeneous and the functionally differentiated MEC appears after
      weaning around acini of the mucous and seromucous glands.
FAU - Ogawa, Y
AU  - Ogawa Y
AD  - Department of Oral Pathology, Osaka University, Faculty of Dentistry, Suita,
      Japan. ogawa@dent.osaka-u.ac.jp
FAU - Yamauchi, S
AU  - Yamauchi S
FAU - Ohnishi, A
AU  - Ohnishi A
FAU - Ito, R
AU  - Ito R
FAU - Ijuhin, N
AU  - Ijuhin N
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Anat Embryol (Berl)
JT  - Anatomy and embryology
JID - 7505194
RN  - 0 (Biomarkers)
RN  - 0 (Muscle Proteins)
SB  - IM
MH  - Animals
MH  - Biomarkers/analysis
MH  - Epithelial Cells/*cytology/metabolism
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Microscopy, Immunoelectron
MH  - Muscle Proteins/immunology/metabolism
MH  - Myocytes, Smooth Muscle/*cytology/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Salivary Glands/*embryology/*growth & development/metabolism
EDAT- 1999/07/29 00:00
MHDA- 2004/10/16 09:00
CRDT- 1999/07/29 00:00
PHST- 1999/07/29 00:00 [pubmed]
PHST- 2004/10/16 09:00 [medline]
PHST- 1999/07/29 00:00 [entrez]
AID - 10.1007/s004290050274 [doi]
PST - ppublish
SO  - Anat Embryol (Berl). 1999 Aug;200(2):215-28. doi: 10.1007/s004290050274.