PMID- 10424490
OWN - NLM
STAT- MEDLINE
DCOM- 19991020
LR  - 20061115
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 20
IP  - 7
DP  - 1999 Jun
TI  - Human bronchoalveolar lavage fluid: two-dimensional gel electrophoresis, amino
      acid microsequencing and identification of major proteins.
PG  - 1634-45
AB  - Although bronchoalveolar lavage has been used as a research and clinical tools
      for more than two decades to investigate the cellular and soluble components of
      the lower respiratory tract, its exact protein composition has never been
      established. In this context, proteins of human bronchoalveolar lavage fluids
      (BALF), obtained by washing the epithelial lining fluid of the lungs with
      phosphate-buffered saline, were analyzed by two-dimensional electrophoresis
      (2-DE) under denaturing and reducing conditions. To characterize the widest
      amount of proteins, an analytical map of human bronchoalveolar lavage fluid
      proteins has been created from a pool of BALF from various patients. The
      resulting map comprises 211 silver-stained spots in the range of pI 3.5-10 and
      molecular mass 5-100 kDa. We identified 182 spots by microsequence analysis and
      by matching with human blood plasma and the Macrophage Like Cell line reference
      2-DE maps available from the SWISS-2DPAGE database. The human bronchoalveolar
      lavage fluid was found to contain 61 different proteins or isoforms thereof. Most
      of the proteins had low molecular masses (< 35 kDa) and rather acidic isoelectric
      points (pI; 4 < pI < 7). The proteins in the lavage either are produced locally
      or originate from plasma. Two unknown proteins were identified and are currently 
      under investigation.
FAU - Wattiez, R
AU  - Wattiez R
AD  - Department of Biological Chemistry, University of Mons-Hainaut, Mons, Belgium.
      rwattiez@ben.vub.ac.be
FAU - Hermans, C
AU  - Hermans C
FAU - Bernard, A
AU  - Bernard A
FAU - Lesur, O
AU  - Lesur O
FAU - Falmagne, P
AU  - Falmagne P
LA  - eng
SI  - GENBANK/AF110731
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Amino Acids)
RN  - 0 (Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Amino Acids/analysis
MH  - Bronchoalveolar Lavage Fluid/*chemistry
MH  - Databases, Factual
MH  - Electrophoresis, Gel, Two-Dimensional/*methods
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Male
MH  - Middle Aged
MH  - Proteins/*chemistry
MH  - Respiratory Tract Diseases/metabolism
MH  - Sequence Analysis/*methods
EDAT- 1999/07/29 10:00
MHDA- 2000/08/12 11:00
CRDT- 1999/07/29 10:00
PHST- 1999/07/29 10:00 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 1999/07/29 10:00 [entrez]
AID - 10.1002/(SICI)1522-2683(19990601)20:7<1634::AID-ELPS1634>3.0.CO;2-J [pii]
AID - 10.1002/(SICI)1522-2683(19990601)20:7<1634::AID-ELPS1634>3.0.CO;2-J [doi]
PST - ppublish
SO  - Electrophoresis. 1999 Jun;20(7):1634-45. doi:
      10.1002/(SICI)1522-2683(19990601)20:7<1634::AID-ELPS1634>3.0.CO;2-J.