PMID- 10422773
OWN - NLM
STAT- MEDLINE
DCOM- 19990928
LR  - 20190624
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 374
IP  - 2
DP  - 1999 Jun 18
TI  - Functional expression of the fractalkine (CX3C) receptor and its regulation by
      lipopolysaccharide in rat microglia.
PG  - 309-13
AB  - Functional expression of CX3CR1, a recently discovered receptor for the chemokine
      fractalkine, was investigated in cultured rat microglia. Reverse transcriptase
      polymerase chain reaction (PCR) experiments show abundant expression of
      fractalkine receptor mRNA in microglia. mRNA expression of fractalkine was
      undetectable in astrocytes and microglia but was very strong in cortical neurons.
      Incubation of microglia with lipopolysaccharide (100 ng/ml) transiently
      suppressed expression of fractalkine receptor mRNA. Fractalkine induced a
      concentration-dependent (10(-10)-10(-8) M) and, at high concentrations,
      oscillatory mobilization of intracellular Ca2+ in microglia The
      concentration-response curve of fractalkine was shifted to the right after 12 h
      incubation with lipopolysaccharide. It is concluded that treatment with endotoxin
      downregulates expression of fractalkine receptor mRNA in rat microglia and
      suppresses the functional response to fractalkine.
FAU - Boddeke, E W
AU  - Boddeke EW
AD  - Department of Medical Physiology, University of Groningen, Netherlands.
      h.w.g.m.boddeke@med.rug.nl
FAU - Meigel, I
AU  - Meigel I
FAU - Frentzel, S
AU  - Frentzel S
FAU - Biber, K
AU  - Biber K
FAU - Renn, L Q
AU  - Renn LQ
FAU - Gebicke-Harter, P
AU  - Gebicke-Harter P
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (CX3C Chemokine Receptor 1)
RN  - 0 (Chemokine CX3CL1)
RN  - 0 (Chemokines, CX3C)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Cx3cl1 protein, rat)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (Receptors, HIV)
RN  - SY7Q814VUP (Calcium)
SB  - IM
SB  - X
MH  - Animals
MH  - CX3C Chemokine Receptor 1
MH  - Calcium/*metabolism
MH  - Cells, Cultured
MH  - Chemokine CX3CL1
MH  - *Chemokines, CX3C
MH  - Chemokines, CXC/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Lipopolysaccharides/*pharmacology
MH  - Male
MH  - Membrane Proteins/*pharmacology
MH  - Microglia/drug effects/*metabolism
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Cytokine/*genetics
MH  - Receptors, HIV/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 1999/07/28 00:00
MHDA- 1999/07/28 00:01
CRDT- 1999/07/28 00:00
PHST- 1999/07/28 00:00 [pubmed]
PHST- 1999/07/28 00:01 [medline]
PHST- 1999/07/28 00:00 [entrez]
AID - S0014-2999(99)00307-6 [pii]
AID - 10.1016/s0014-2999(99)00307-6 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 1999 Jun 18;374(2):309-13. doi: 10.1016/s0014-2999(99)00307-6.