PMID- 10422644
OWN - NLM
STAT- MEDLINE
DCOM- 19990916
LR  - 20190624
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 374
IP  - 1
DP  - 1999 Jun 11
TI  - Neuropeptide Y-induced contraction is mediated by neuropeptide Y Y2 and Y4
      receptors in the rat colon.
PG  - 85-91
AB  - Ascending and descending segments of the rat colon were studied to analyze their 
      contractile responses to neuropeptide Y and related peptides. These responses are
      (a) completely eliminated by tetrodotoxin (1 microM), (b) reduced to a variable
      extent (20 to 60%) by atropine (1 microM) and (c) not modified by indomethacin,
      diphenhydramine or methysergide. The order of potency of agonists for peptides
      related to neuropeptide Y was as follows: human pancreatic polypeptide = rat
      pancreatic polypeptide > peptide YY = peptide YY-(3-36) =
      [Leu31,Pro34]neuropeptide Y > neuropeptide Y-(2-36) > C2-neuropeptide Y =
      neuropeptide Y > neuropeptide Y-(13-36), with minor differences observed between 
      the two parts of the colon. This selectivity pattern does not correspond to the
      profile of any known cloned neuropeptide Y receptors. BIBP3226, a selective
      antagonist for the neuropeptide Y Y1 receptor sub-type, was found to be inactive,
      while a neuropeptide Y Y2 receptor antagonist, T4-[NPY-(33-36)]4, reduced the
      effects of neuropeptide Y, peptide YY, peptide YY-(3-36) and C2-neuropeptide Y
      without affecting those of human pancreatic polypeptide, rat pancreatic
      polypeptide and [Leu31,Pro34]neuropeptide Y. JCF 104 (compound 28), a putative
      neuropeptide Y Y5 receptor antagonist, showed no effect or a weak inhibition of
      human pancreatic polypeptide or [Leu31,Pro34]neuropeptide Y-induced contraction. 
      Taken together, these data suggest that: (1) at least two neuropeptide Y receptor
      types are present in the rat colon autonomic nerve terminals and modulate the
      release of acetylcholine and possibly other transmitters; (2) a proportion of the
      receptors mediating the contractile response of the rat colon (especially
      descending part) to neuropeptide Y and related peptides appears to be of the Y2
      type and (3) the significant portion of the response is mediated by a receptor
      which is insensitive to neuropeptide Y Y1, Y2 and to neuropeptide Y Y5 receptor
      antagonists. This receptor behaves as a neuropeptide Y Y4 receptor sub-type and
      appears to be located on enteric nerves.
FAU - Pheng, L H
AU  - Pheng LH
AD  - Department of Pharmacology, Medical School, Universite de Sherbrooke, Quebec,
      Canada.
FAU - Perron, A
AU  - Perron A
FAU - Quirion, R
AU  - Quirion R
FAU - Cadieux, A
AU  - Cadieux A
FAU - Fauchere, J L
AU  - Fauchere JL
FAU - Dumont, Y
AU  - Dumont Y
FAU - Regoli, D
AU  - Regoli D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Neuropeptide Y)
RN  - 0 (Receptors, Neuropeptide Y)
RN  - 0 (neuropeptide Y2 receptor)
RN  - 106388-42-5 (Peptide YY)
RN  - 55F8T1GN3X (neuropeptide Y4 receptor)
SB  - IM
MH  - Animals
MH  - Colon/*drug effects/physiology
MH  - Female
MH  - Male
MH  - Muscle Contraction/drug effects
MH  - Neuropeptide Y/*pharmacology
MH  - Peptide YY/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Neuropeptide Y/*drug effects/physiology
EDAT- 1999/07/28 00:00
MHDA- 1999/07/28 00:01
CRDT- 1999/07/28 00:00
PHST- 1999/07/28 00:00 [pubmed]
PHST- 1999/07/28 00:01 [medline]
PHST- 1999/07/28 00:00 [entrez]
AID - S0014-2999(99)00296-4 [pii]
AID - 10.1016/s0014-2999(99)00296-4 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 1999 Jun 11;374(1):85-91. doi: 10.1016/s0014-2999(99)00296-4.