PMID- 10421840
OWN - NLM
STAT- MEDLINE
DCOM- 19990818
LR  - 20191103
IS  - 1356-9597 (Print)
IS  - 1356-9597 (Linking)
VI  - 4
IP  - 5
DP  - 1999 May
TI  - Molecular cloning and characterization of a novel member of the MAP kinase
      superfamily.
PG  - 299-309
AB  - BACKGROUND: Members of the MAP kinase superfamily play important roles in a wide 
      variety of signal transduction pathways, and several members have been
      identified. However, the diversity and complexity of cellular responses in
      mammalian systems may imply existence of hitherto unidentified members of the MAP
      kinase superfamily. RESULTS: We report the molecular cloning and characterization
      of a novel member of the MAP kinase superfamily. We isolated full-length mouse
      and human cDNAs that encode complete open reading frames of a novel protein
      kinase, termed MOK. MOK consists of 419 (human) and 420 (mouse) amino acids, with
      a calculated molecular weight of 48kDa. MOK contains all of the protein
      serine/threonine kinase consensus motifs and shows a modest similarity to members
      of the MAP kinase superfamily and MAK and MAK-related kinase (MRK). In addition, 
      MOK possesses a Thr-Glu-Tyr (TEY) motif in the activation loop domain, as do
      classical MAP kinases. MOK is widely expressed in normal tissues and organs and
      localizes to the cytoplasm. MOK is able to phosphorylate several known MAP kinase
      substrates and to undergo autophosphorylation. A mutation in the TEY motif to AEF
      abolished the kinase activity of MOK, and the treatment of cells with a
      phosphatase inhibitor, okadaic acid, enhanced the kinase activity of MOK,
      suggesting the existence of an upstream kinase. Phorbol ester TPA was found to
      stimulate the kinase activity of MOK, whereas serum stimulation, osmotic shock,
      or anisomycin treatment did not significantly activate MOK. CONCLUSION: These
      results indicate that MOK is distantly related to members of known subfamilies of
      the MAP kinase superfamily and can therefore be classified as a novel member.
FAU - Miyata, Y
AU  - Miyata Y
AD  - Department of Biophysics, Graduate School of Science, Kyoto University, Sakyo-ku,
      Kyoto 606-8502, Japan.
FAU - Akashi, M
AU  - Akashi M
FAU - Nishida, E
AU  - Nishida E
LA  - eng
SI  - GENBANK/AB022694
SI  - GENBANK/AB022695
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Genes Cells
JT  - Genes to cells : devoted to molecular & cellular mechanisms
JID - 9607379
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor for Advanced Glycation End Products)
RN  - 2ZD004190S (Threonine)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.22 (Mok protein, mouse)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - IY9XDZ35W2 (Glucose)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Blotting, Northern
MH  - COS Cells
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*genetics/metabolism
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 14/genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry/genetics
MH  - Enzyme Activation/drug effects
MH  - Expressed Sequence Tags
MH  - Female
MH  - Gene Expression
MH  - Glucose/chemistry
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mitogen-Activated Protein Kinases
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Protein Conformation
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptor for Advanced Glycation End Products
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Threonine/chemistry/metabolism
MH  - Tissue Distribution
MH  - Tyrosine/chemistry/metabolism
EDAT- 1999/07/28 00:00
MHDA- 1999/07/28 00:01
CRDT- 1999/07/28 00:00
PHST- 1999/07/28 00:00 [pubmed]
PHST- 1999/07/28 00:01 [medline]
PHST- 1999/07/28 00:00 [entrez]
AID - gtc261 [pii]
AID - 10.1046/j.1365-2443.1999.00261.x [doi]
PST - ppublish
SO  - Genes Cells. 1999 May;4(5):299-309. doi: 10.1046/j.1365-2443.1999.00261.x.