PMID- 10421839
OWN - NLM
STAT- MEDLINE
DCOM- 19990818
LR  - 20191103
IS  - 1356-9597 (Print)
IS  - 1356-9597 (Linking)
VI  - 4
IP  - 5
DP  - 1999 May
TI  - Involvement of CRM1, a nuclear export receptor, in mRNA export in mammalian cells
      and fission yeast.
PG  - 291-7
AB  - BACKGROUND: CRM1, an evolutionarily conserved protein, was shown to be a receptor
      for leucine-rich nuclear export signal (NES)-dependent protein transport. In
      lower eukaryotes CRM1 is reported to be required for the export of mRNA, however,
      involvement of the NES-dependent transport pathway in mRNA export in higher
      eukaryotes has not been established. RESULTS: We have found that treatment of
      mammalian cells with leptomycin B (LMB), a specific inhibitor of CRM1, induces
      the nuclear accumulation of endogenous mRNA, probably due to the inhibition of
      its export. In fission yeasts, the nuclear accumulation of mRNA also occurred in 
      cells treated with LMB or in a temperature-sensitive crm1 mutant at a restrictive
      temperature. A synthetic mRNA that was injected into the nucleus of mammalian
      cultured cells was exported from the nucleus within 5 h. This export was
      inhibited by both wheat germ agglutinin and a temperature of 4 degrees C.
      Importantly, this mRNA export was inhibited by LMB or by an excess amount of the 
      NES peptide-conjugates. LMB treatment, on the other hand, rapidly induced the
      nuclear entry of RanBP1, a factor involved in the active nucleocytoplasmic
      transport, although the treatment did not interfere with a nuclear localization
      signal-dependent transport system within 7 h. CONCLUSION: These results suggest
      that CRM1 is involved in mRNA export in eukaryotic cells.
FAU - Watanabe, M
AU  - Watanabe M
AD  - Department of Biophysics, Graduate School of Science, Kyoto University, Sakyo-ku,
      Kyoto 606-8502, Japan.
FAU - Fukuda, M
AU  - Fukuda M
FAU - Yoshida, M
AU  - Yoshida M
FAU - Yanagida, M
AU  - Yanagida M
FAU - Nishida, E
AU  - Nishida E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Genes Cells
JT  - Genes to cells : devoted to molecular & cellular mechanisms
JID - 9607379
RN  - 0 (Antifungal Agents)
RN  - 0 (Carrier Proteins)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Karyopherins)
RN  - 0 (Nuclear Localization Signals)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (exportin 1 protein)
RN  - 0 (ran-binding protein 1)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 9006-59-1 (Ovalbumin)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (ran GTP-Binding Protein)
RN  - Y031I2N1EO (leptomycin B)
SB  - IM
MH  - Animals
MH  - Antifungal Agents/pharmacology
MH  - Biological Transport
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cattle
MH  - Cell Line
MH  - Cell Nucleus/drug effects/metabolism
MH  - Cytoplasm/drug effects/metabolism
MH  - Fatty Acids, Unsaturated/pharmacology
MH  - Fluorescent Dyes
MH  - GTP-Binding Proteins/drug effects/metabolism
MH  - *Karyopherins
MH  - Microinjections
MH  - Mutation
MH  - Nuclear Localization Signals/drug effects
MH  - Nuclear Proteins/drug effects/genetics/*metabolism
MH  - Ovalbumin/metabolism
MH  - RNA, Messenger/administration & dosage/drug effects/*metabolism
MH  - Rats
MH  - *Receptors, Cytoplasmic and Nuclear
MH  - Ribonucleoproteins/metabolism
MH  - Schizosaccharomyces/cytology/*metabolism
MH  - Serum Albumin, Bovine/metabolism
MH  - Temperature
MH  - *ran GTP-Binding Protein
EDAT- 1999/07/28 00:00
MHDA- 1999/07/28 00:01
CRDT- 1999/07/28 00:00
PHST- 1999/07/28 00:00 [pubmed]
PHST- 1999/07/28 00:01 [medline]
PHST- 1999/07/28 00:00 [entrez]
AID - gtc259 [pii]
AID - 10.1046/j.1365-2443.1999.00259.x [doi]
PST - ppublish
SO  - Genes Cells. 1999 May;4(5):291-7. doi: 10.1046/j.1365-2443.1999.00259.x.