PMID- 10421633
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20190516
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 13
IP  - 14
DP  - 1999 Jul 15
TI  - SNAP(c): a core promoter factor with a built-in DNA-binding damper that is
      deactivated by the Oct-1 POU domain.
PG  - 1807-21
AB  - snRNA gene transcription is activated in part by recruitment of SNAP(c) to the
      core promoter through protein-protein contacts with the POU domain of the
      enhancer-binding factor Oct-1. We show that a mini-SNAP(c) consisting of a subset
      of SNAP(c) subunits is capable of directing both RNA polymerase II (Pol II) and
      Pol III snRNA gene transcription. Mini-SNAP(c) cannot be recruited by Oct-1, but 
      binds as efficiently to the promoter as SNAP(c) together with Oct-1 and directs
      activated RNA Pol III transcription. Thus, SNAP(c) represses its own binding to
      DNA, and repression is relieved by interactions with the Oct-1 POU domain that
      promote cooperative binding. We have shown previously that TBP also represses its
      own binding, and in that case repression is relieved by cooperative interactions 
      with SNAP(c). This may represent a general mechanism to ensure that core
      promoter-binding factors, which have strikingly slow off-rates, are recruited
      specifically to promoter sequences rather than to cryptic-binding sites in the
      genome.
FAU - Mittal, V
AU  - Mittal V
AD  - Howard Hughes Medical Institute and Cold Spring Harbor Laboratory, Cold Spring
      Harbor, New York 11724 USA.
FAU - Ma, B
AU  - Ma B
FAU - Hernandez, N
AU  - Hernandez N
LA  - eng
GR  - R01 GM038810/GM/NIGMS NIH HHS/United States
GR  - GM38810/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (POU Domain Factors)
RN  - 0 (RNA, Small Nuclear)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.7.- (RNA Polymerase II)
RN  - EC 2.7.7.6 (RNA Polymerase III)
SB  - IM
MH  - DNA-Binding Proteins/*antagonists & inhibitors/*metabolism
MH  - Humans
MH  - POU Domain Factors
MH  - *Promoter Regions, Genetic
MH  - RNA Polymerase II/metabolism
MH  - RNA Polymerase III/metabolism
MH  - RNA, Small Nuclear/genetics
MH  - Transcription Factors/*metabolism
MH  - Transcription, Genetic
PMC - PMC316888
EDAT- 1999/07/27 00:00
MHDA- 1999/07/27 00:01
CRDT- 1999/07/27 00:00
PHST- 1999/07/27 00:00 [pubmed]
PHST- 1999/07/27 00:01 [medline]
PHST- 1999/07/27 00:00 [entrez]
AID - 10.1101/gad.13.14.1807 [doi]
PST - ppublish
SO  - Genes Dev. 1999 Jul 15;13(14):1807-21. doi: 10.1101/gad.13.14.1807.