PMID- 10421629
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20190516
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 13
IP  - 14
DP  - 1999 Jul 15
TI  - Wnt-induced dephosphorylation of axin releases beta-catenin from the axin
      complex.
PG  - 1768-73
AB  - The stabilization of beta-catenin is a key regulatory step during cell fate
      changes and transformations to tumor cells. Several interacting proteins,
      including Axin, APC, and the protein kinase GSK-3beta are implicated in
      regulating beta-catenin phosphorylation and its subsequent degradation. Wnt
      signaling stabilizes beta-catenin, but it was not clear whether and how Wnt
      signaling regulates the beta-catenin complex. Here we show that Axin is
      dephosphorylated in response to Wnt signaling. The dephosphorylated Axin binds
      beta-catenin less efficiently than the phosphorylated form. Thus, Wnt signaling
      lowers Axin's affinity for beta-catenin, thereby disengaging beta-catenin from
      the degradation machinery.
FAU - Willert, K
AU  - Willert K
AD  - Howard Hughes Medical Institute (HHMI) and Department of Developmental Biology,
      Stanford University School of Medicine, Stanford, California 94305 USA.
FAU - Shibamoto, S
AU  - Shibamoto S
FAU - Nusse, R
AU  - Nusse R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Axin Protein)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Wnt Proteins)
RN  - 0 (Zebrafish Proteins)
RN  - 0 (beta Catenin)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.26 (Glycogen Synthase Kinase 3)
SB  - IM
MH  - Axin Protein
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - Cell Line
MH  - Cytoskeletal Proteins/*metabolism
MH  - Glycogen Synthase Kinase 3
MH  - Phosphorylation
MH  - Protein Binding
MH  - Proteins/*metabolism
MH  - Proto-Oncogene Proteins/*metabolism
MH  - *Repressor Proteins
MH  - Signal Transduction
MH  - *Trans-Activators
MH  - Wnt Proteins
MH  - *Zebrafish Proteins
MH  - beta Catenin
PMC - PMC316878
EDAT- 1999/07/27 00:00
MHDA- 1999/07/27 00:01
CRDT- 1999/07/27 00:00
PHST- 1999/07/27 00:00 [pubmed]
PHST- 1999/07/27 00:01 [medline]
PHST- 1999/07/27 00:00 [entrez]
AID - 10.1101/gad.13.14.1768 [doi]
PST - ppublish
SO  - Genes Dev. 1999 Jul 15;13(14):1768-73. doi: 10.1101/gad.13.14.1768.