PMID- 10419521
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 31
DP  - 1999 Jul 30
TI  - Cloning and characterization of the EAP30 subunit of the ELL complex that confers
      derepression of transcription by RNA polymerase II.
PG  - 21981-5
AB  - The product of the human oncogene ELL encodes an RNA polymerase II transcription 
      factor that undergoes frequent translocation in acute myeloid leukemia (AML). In 
      addition to its elongation activity, ELL contains a novel type of RNA polymerase 
      II interaction domain that is capable of repressing polymerase activity in
      promoter-specific transcription. Remarkably, the ELL translocation that is found 
      in patients with AML results in the deletion of exactly this functional domain.
      Here we report that the EAP30 subunit of the ELL complex has sequence homology to
      the Saccharomyces cerevisiae SNF8, whose genetic analysis suggests its
      involvement in the derepression of gene expression. Remarkably, EAP30 can
      interact with ELL and derepress ELL's inhibitory activity in vitro. This finding 
      may reveal a key role for EAP30 in the pathogenesis of human leukemia.
FAU - Schmidt, A E
AU  - Schmidt AE
AD  - Edward A. Doisy Department of Biochemistry, Saint Louis University School of
      Medicine, Saint Louis, Missouri 63104, USA.
FAU - Miller, T
AU  - Miller T
FAU - Schmidt, S L
AU  - Schmidt SL
FAU - Shiekhattar, R
AU  - Shiekhattar R
FAU - Shilatifard, A
AU  - Shilatifard A
LA  - eng
SI  - GENBANK/AF156102
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (ELL protein, human)
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - 0 (Fungal Proteins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Peptide Elongation Factors)
RN  - 0 (Recombinant Proteins)
RN  - 0 (SNF8 protein, S cerevisiae)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Transcriptional Elongation Factors)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/chemistry/*genetics/*metabolism
MH  - Endosomal Sorting Complexes Required for Transport
MH  - Enzyme Repression
MH  - Fungal Proteins/chemistry
MH  - Humans
MH  - Leukemia, Myeloid, Acute/genetics/metabolism
MH  - Macromolecular Substances
MH  - Molecular Sequence Data
MH  - *Neoplasm Proteins
MH  - Oncogenes
MH  - *Peptide Elongation Factors
MH  - *Promoter Regions, Genetic
MH  - RNA Polymerase II/biosynthesis/*metabolism
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Saccharomyces cerevisiae/genetics
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Transcription Factors/chemistry/*genetics/*metabolism
MH  - *Transcription, Genetic
MH  - Transcriptional Elongation Factors
EDAT- 1999/07/27 00:00
MHDA- 1999/07/27 00:01
CRDT- 1999/07/27 00:00
PHST- 1999/07/27 00:00 [pubmed]
PHST- 1999/07/27 00:01 [medline]
PHST- 1999/07/27 00:00 [entrez]
AID - 10.1074/jbc.274.31.21981 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jul 30;274(31):21981-5. doi: 10.1074/jbc.274.31.21981.