PMID- 10419454
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 31
DP  - 1999 Jul 30
TI  - Pleckstrin 2, a widely expressed paralog of pleckstrin involved in actin
      rearrangement.
PG  - 21515-8
AB  - We have identified a cDNA for pleckstrin 2 that is 39% identical and 65%
      homologous to the original pleckstrin. Like the original pleckstrin 1, this
      protein contains a pleckstrin homology (PH) domain at each end of the molecule as
      well as a DEP (Dishevelled, Egl-10, and pleckstrin) domain in the intervening
      sequence. A Northern blot probed with the full-length cDNA reveals that this
      homolog is ubiquitously expressed and is most abundant in the thymus, large
      bowel, small bowel, stomach, and prostate. Unlike pleckstrin 1, this newly
      discovered protein does not contain obvious sites of PKC phosphorylation, and in 
      transfected Cos-7 cells, it is a poor substrate for phosphorylation, even after
      PMA stimulation. Cells expressing pleckstrin 2 undergo a dramatic shape change
      associated with actin rearrangement, including a loss of central F-actin and a
      redistribution of actin toward the cell cortex. Overexpression of pleckstrin 2
      causes large lamellipodia and peripheral ruffle formation. A variant of
      pleckstrin 2 lacking both PH domains still had some membrane binding but did not 
      efficiently induce lamellipodia, suggesting that the PH domains of pleckstrin 2
      contribute to lamellipodia formation. This work describes a novel, widely
      expressed, membrane-associating protein and suggests that pleckstrin 2 may help
      orchestrate cytoskeletal arrangement.
FAU - Hu, M H
AU  - Hu MH
AD  - Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania
      19104, USA.
FAU - Bauman, E M
AU  - Bauman EM
FAU - Roll, R L
AU  - Roll RL
FAU - Yeilding, N
AU  - Yeilding N
FAU - Abrams, C S
AU  - Abrams CS
LA  - eng
SI  - GENBANK/AF157600
GR  - P01 HL40387/HL/NHLBI NIH HHS/United States
GR  - P50 HL54500/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Actins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Proteins)
RN  - 0 (PLEK2 protein, human)
RN  - 0 (Plek2 protein, mouse)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Actins/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells
MH  - Cell Size/*physiology
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - DNA, Complementary
MH  - Female
MH  - Humans
MH  - Male
MH  - Mammals
MH  - Membrane Proteins/chemistry/genetics/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Phosphorylation
MH  - Polymerase Chain Reaction
MH  - Protein Kinase C/metabolism
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Transfection
MH  - src Homology Domains
EDAT- 1999/07/27 00:00
MHDA- 1999/07/27 00:01
CRDT- 1999/07/27 00:00
PHST- 1999/07/27 00:00 [pubmed]
PHST- 1999/07/27 00:01 [medline]
PHST- 1999/07/27 00:00 [entrez]
AID - 10.1074/jbc.274.31.21515 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jul 30;274(31):21515-8. doi: 10.1074/jbc.274.31.21515.