PMID- 10419450
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 31
DP  - 1999 Jul 30
TI  - LEKTI, a novel 15-domain type of human serine proteinase inhibitor.
PG  - 21499-502
AB  - Proteinase inhibitors are important negative regulators of proteinase action in
      vivo. We have succeeded in isolating two previously unknown polypeptides (HF6478 
      and HF7665) from human blood filtrate that are parts of a larger precursor
      protein containing two typical Kazal-type serine proteinase inhibitor motifs. The
      entire precursor protein, as deduced from the nucleotide sequence of the cloned
      cDNA, exhibits 15 potential inhibitory domains, including the Kazal-type domains,
      HF6478, HF7665, and 11 additional similar domains. An inhibitory effect of HF7665
      on trypsin activity is demonstrated. Because all of the 13 HF6478- and
      HF7665-related domains share partial homology to the typical Kazal-type domain
      but lack one of the three conserved disulfide bonds, they may represent a novel
      type of serine proteinase inhibitor. The gene encoding the multidomain proteinase
      inhibitor, which we have termed LEKTI, was localized on human chromosome 5q31-32.
      As shown by reverse transcriptase-polymerase chain reaction and Northern blot
      analysis, it is expressed in the thymus, vaginal epithelium, Bartholin's glands, 
      oral mucosa, tonsils, and the parathyroid glands. From these results, we assume
      that LEKTI may play a role in anti-inflammatory and/or antimicrobial protection
      of mucous epithelia.
FAU - Magert, H J
AU  - Magert HJ
AD  - Lower Saxony Institute for Peptide Research, Feodor-Lynen-Strasse 31, D-30 625
      Hannover, Germany. HJ-Maegert@gmx.de
FAU - Standker, L
AU  - Standker L
FAU - Kreutzmann, P
AU  - Kreutzmann P
FAU - Zucht, H D
AU  - Zucht HD
FAU - Reinecke, M
AU  - Reinecke M
FAU - Sommerhoff, C P
AU  - Sommerhoff CP
FAU - Fritz, H
AU  - Fritz H
FAU - Forssmann, W G
AU  - Forssmann WG
LA  - eng
SI  - GENBANK/AF086524
SI  - GENBANK/AJ228139
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Proteinase Inhibitory Proteins, Secretory)
RN  - 0 (Recombinant Proteins)
RN  - 0 (SPINK5 protein, human)
RN  - 0 (Serine Peptidase Inhibitor Kazal-Type 5)
RN  - 0 (Serine Proteinase Inhibitors)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - *Carrier Proteins
MH  - Chromatography, Gel
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - DNA, Complementary
MH  - Humans
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Proteinase Inhibitory Proteins, Secretory
MH  - Recombinant Proteins/chemistry/isolation & purification/pharmacology
MH  - Serine Peptidase Inhibitor Kazal-Type 5
MH  - Serine Proteinase Inhibitors/*blood/chemistry/genetics
MH  - Trypsin/*metabolism
MH  - Ultrafiltration
EDAT- 1999/07/27 00:00
MHDA- 1999/07/27 00:01
CRDT- 1999/07/27 00:00
PHST- 1999/07/27 00:00 [pubmed]
PHST- 1999/07/27 00:01 [medline]
PHST- 1999/07/27 00:00 [entrez]
AID - 10.1074/jbc.274.31.21499 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jul 30;274(31):21499-502. doi: 10.1074/jbc.274.31.21499.