PMID- 10417378
OWN - NLM
STAT- MEDLINE
DCOM- 19990812
LR  - 20220321
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 285
IP  - 5427
DP  - 1999 Jul 23
TI  - Light-dependent sequestration of TIMELESS by CRYPTOCHROME.
PG  - 553-6
AB  - Most organisms have circadian clocks consisting of negative feedback loops of
      gene regulation that facilitate adaptation to cycles of light and darkness. In
      this study, CRYPTOCHROME (CRY), a protein involved in circadian photoperception
      in Drosophila, is shown to block the function of PERIOD/TIMELESS (PER/TIM)
      heterodimeric complexes in a light-dependent fashion. TIM degradation does not
      occur under these conditions; thus, TIM degradation is uncoupled from abrogation 
      of its function by light. CRY and TIM are part of the same complex and directly
      interact in yeast in a light-dependent fashion. PER/TIM and CRY influence the
      subcellular distribution of these protein complexes, which reside primarily in
      the nucleus after the perception of a light signal. Thus, CRY acts as a circadian
      photoreceptor by directly interacting with core components of the circadian
      clock.
FAU - Ceriani, M F
AU  - Ceriani MF
AD  - Department of Cell Biology and NSF Center for Biological Timing, The Scripps
      Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
FAU - Darlington, T K
AU  - Darlington TK
FAU - Staknis, D
AU  - Staknis D
FAU - Mas, P
AU  - Mas P
FAU - Petti, A A
AU  - Petti AA
FAU - Weitz, C J
AU  - Weitz CJ
FAU - Kay, S A
AU  - Kay SA
LA  - eng
GR  - MH-51573/MH/NIMH NIH HHS/United States
GR  - MH-59943/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Cryptochromes)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Eye Proteins)
RN  - 0 (Flavoproteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (PER protein, Drosophila)
RN  - 0 (Period Circadian Proteins)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (cry protein, Drosophila)
RN  - 0 (tim protein, Drosophila)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
CIN - Science. 1999 Jul 23;285(5427):506-7. PMID: 10447476
MH  - Animals
MH  - *Biological Clocks
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - *Circadian Rhythm
MH  - Cryptochromes
MH  - Cytoplasm/metabolism
MH  - Darkness
MH  - Dimerization
MH  - Drosophila
MH  - *Drosophila Proteins
MH  - *Eye Proteins
MH  - Flavoproteins/genetics/*metabolism
MH  - Green Fluorescent Proteins
MH  - Insect Proteins/genetics/*metabolism
MH  - *Light
MH  - Luminescent Proteins
MH  - Mutation
MH  - Nuclear Proteins/genetics/metabolism
MH  - Period Circadian Proteins
MH  - *Photoreceptor Cells, Invertebrate
MH  - Receptors, G-Protein-Coupled
MH  - Recombinant Fusion Proteins/metabolism
MH  - Transfection
MH  - Yeasts/genetics/metabolism
EDAT- 1999/07/27 00:00
MHDA- 1999/07/27 00:01
CRDT- 1999/07/27 00:00
PHST- 1999/07/27 00:00 [pubmed]
PHST- 1999/07/27 00:01 [medline]
PHST- 1999/07/27 00:00 [entrez]
AID - 7695 [pii]
AID - 10.1126/science.285.5427.553 [doi]
PST - ppublish
SO  - Science. 1999 Jul 23;285(5427):553-6. doi: 10.1126/science.285.5427.553.