PMID- 10417349
OWN - NLM
STAT- MEDLINE
DCOM- 19990916
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 341 ( Pt 3)
DP  - 1999 Aug 1
TI  - Molecular and functional analysis of mouse decay accelerating factor (CD55).
PG  - 821-9
AB  - Molecular cloning of mouse decay accelerating factor (DAF; CD55) predicted two
      forms of the molecule, one transmembrane (TM) and the other
      glycosylphosphatidylinositol (GPI)-anchored; these are encoded by separate genes 
      termed Daf-GPI and Daf-TM. In the present study several additional isoforms of
      mouse DAF, generated by alternative splicing from these genes, are described.
      Northern-blot analysis of RNA and reverse transcriptase-PCR from various tissues 
      indicated that spleen and testis expressed high levels of DAF, which comprised
      several species. These species were cloned and sequence analysis revealed various
      novel forms in addition to those previously reported. Two novel forms were
      derived from the Daf-TM gene but the transmembrane sequence defined previously
      was replaced by a unique GPI-anchor addition sequence; one clone also had part of
      the serine/threonine/proline (STP) region deleted. A third clone, encoding a
      transmembrane protein, was also derived from this gene but the entire STP region 
      was deleted. A fourth clone, derived from the Daf-GPI gene, contained a novel
      C-terminal sequence, suggestive of a secreted form of the protein. Two DAF cDNAs 
      (TM and GPI-anchored) were stably expressed in Chinese hamster ovary cells. When 
      these cells were attacked with mouse or rat complement and analysed for C3b
      deposition, DAF-transfected cells had greatly reduced C3b deposition compared
      with controls. Transfection with DAF also conferred protection from complement in
      a cell-lysis assay, and a soluble, recombinant form of mouse DAF inhibited
      complement in a haemolytic assay.
FAU - Harris, C L
AU  - Harris CL
AD  - Department of Medical Biochemistry, University of Wales College of Medicine,
      Heath Park, Cardiff CF14 4XN, U.K.
FAU - Rushmere, N K
AU  - Rushmere NK
FAU - Morgan, B P
AU  - Morgan BP
LA  - eng
SI  - GENBANK/AF143539
SI  - GENBANK/AF143540
SI  - GENBANK/AF143541
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (CD55 Antigens)
RN  - 0 (DNA Primers)
RN  - 0 (Protein Sorting Signals)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - CD55 Antigens/*genetics/*metabolism
MH  - CHO Cells
MH  - Cricetinae
MH  - DNA Primers
MH  - Mice
MH  - Molecular Sequence Data
MH  - Protein Sorting Signals/genetics
MH  - Rats
MH  - Sequence Homology, Amino Acid
PMC - PMC1220423
EDAT- 1999/07/27 00:00
MHDA- 1999/07/27 00:01
CRDT- 1999/07/27 00:00
PHST- 1999/07/27 00:00 [pubmed]
PHST- 1999/07/27 00:01 [medline]
PHST- 1999/07/27 00:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Aug 1;341 ( Pt 3):821-9.