PMID- 10417313
OWN - NLM
STAT- MEDLINE
DCOM- 19990916
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 341 ( Pt 3)
DP  - 1999 Aug 1
TI  - Functional co-operation between the subunits in heterodimeric platelet-derived
      growth factor receptor complexes.
PG  - 523-8
AB  - To determine the importance of the phosphorylation capacity of receptor kinase as
      well as the ability to serve as docking sites for SH2-domain-containing signal
      transduction molecules, we established pig aortic endothelial cell lines stably
      expressing kinase-active platelet-derived growth factor (PDGF) alpha-receptors
      together with kinase-inactive beta-receptors, and vice versa. After stimulation
      with PDGF-AB, heterodimeric receptor complexes were formed in which the
      kinase-inactive receptor was phosphorylated by the kinase-active receptor,
      although less efficiently than in heterodimers of wild-type receptors. The
      kinase-active receptor was only minimally phosphorylated. Thus the
      phosphorylation within the receptor dimer occurred in trans between the
      components. Analyses of the abilities of heterodimeric receptor complexes of one 
      kinase-active and one kinase-inactive receptor to mediate mitogenicity,
      chemotaxis and activation of mitogen-activated protein kinase revealed less
      efficient effects than those of heterodimers of wild-type receptors. Importantly,
      however, the fact that signalling capacities were retained illustrates a
      functional co-operation between the two receptor molecules in the dimer, where
      one receptor provides a functional kinase and the other acts as a substrate and
      provides docking sites for downstream signalling molecules.
FAU - Emaduddin, M
AU  - Emaduddin M
AD  - Ludwig Institute for Cancer Research, Box 595, Biomedical Center, S-751 24
      Uppsala, Sweden.
FAU - Ekman, S
AU  - Ekman S
FAU - Ronnstrand, L
AU  - Ronnstrand L
FAU - Heldin, C H
AU  - Heldin CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (DNA Primers)
RN  - 0 (Mitogens)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.10.1 (Receptors, Platelet-Derived Growth Factor)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - Cell Line
MH  - Cell Movement/drug effects
MH  - DNA Primers
MH  - Dimerization
MH  - Enzyme Activation
MH  - Mitogens/pharmacology
MH  - Peptide Mapping
MH  - Phosphorylation
MH  - Platelet-Derived Growth Factor/pharmacology
MH  - Receptors, Platelet-Derived Growth Factor/*metabolism
MH  - *Signal Transduction
MH  - Swine
MH  - Tyrosine/metabolism
PMC - PMC1220387
EDAT- 1999/07/27 00:00
MHDA- 1999/07/27 00:01
CRDT- 1999/07/27 00:00
PHST- 1999/07/27 00:00 [pubmed]
PHST- 1999/07/27 00:01 [medline]
PHST- 1999/07/27 00:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Aug 1;341 ( Pt 3):523-8.