PMID- 10417266
OWN - NLM
STAT- MEDLINE
DCOM- 19990913
LR  - 20081121
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 260
IP  - 2
DP  - 1999 Aug 1
TI  - Extensive sequence divergence and phylogenetic relationships between the
      fusogenic and nonfusogenic orthoreoviruses: a species proposal.
PG  - 316-28
AB  - The orthoreoviruses can be divided into subgroups based on either their
      restricted host range or the unusual ability of certain members of this group of 
      nonenveloped viruses to induce cell-cell fusion from within. Phylogenetic
      relationships cannot be inferred based on these biological properties because
      fusogenic reoviruses are present in both the avian and mammalian subgroups. To
      address this issue, the complete nucleotide sequences of the three S-class genome
      segments encoding the major sigma-class core, outer capsid, and nonstructural
      proteins of four fusogenic reoviruses were determined and used to establish the
      phylogeny of the orthoreoviruses. The viruses analysed included two strains of
      avian reovirus and the only known fusogenic mammalian reoviruses, Nelson Bay
      virus and baboon reovirus. Comparative sequence analysis of these fusogenic
      reoviruses and the prototypical nonfusogenic mammalian reoviruses indicated a
      highly diverged genus with both conserved and unique sequence-predicted
      structural motifs in the major sigma-class proteins. Phylogenetic analysis
      provided the basis for the first taxonomic subdivision of the orthoreoviruses
      into species classes based on inferred evolutionary relationships. It is proposed
      that the orthoreoviruses consist of at least four species that separate into
      three clades. The nonfusogenic mammalian reovirus species represent a single
      clade, and the fusogenic reoviruses separate into two distinct clades. The first 
      clade of fusogenic reoviruses contains the avian reovirus- and Nelson Bay
      virus-type species, with the second clade being occupied by the single baboon
      reovirus isolate that represents a fourth orthoreovirus species.
CI  - Copyright 1999 Academic Press.
FAU - Duncan, R
AU  - Duncan R
AD  - Department of Microbiology and Immunology, Faculty of Medicine, Halifax, Nova
      Scotia, B3H 4H7, Canada. roy.duncan@dal.ca
LA  - eng
SI  - GENBANK/AF059716
SI  - GENBANK/AF059717
SI  - GENBANK/AF059718
SI  - GENBANK/AF059719
SI  - GENBANK/AF059720
SI  - GENBANK/AF059721
SI  - GENBANK/AF059722
SI  - GENBANK/AF059723
SI  - GENBANK/AF059724
SI  - GENBANK/AF059725
SI  - GENBANK/AF059726
SI  - GENBANK/AF059727
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - *Cell Fusion
MH  - Conserved Sequence/genetics
MH  - *Evolution, Molecular
MH  - Genes, Viral/genetics
MH  - Genetic Variation/*genetics
MH  - Genome, Viral
MH  - Molecular Sequence Data
MH  - Nucleocapsid Proteins/chemistry/genetics
MH  - Orthoreovirus/*classification/*genetics/growth & development/physiology
MH  - Phenotype
MH  - *Phylogeny
MH  - Protein Structure, Secondary
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Viral Nonstructural Proteins/chemistry/genetics
EDAT- 1999/07/27 10:00
MHDA- 2000/08/06 11:00
CRDT- 1999/07/27 10:00
PHST- 1999/07/27 10:00 [pubmed]
PHST- 2000/08/06 11:00 [medline]
PHST- 1999/07/27 10:00 [entrez]
AID - 10.1006/viro.1999.9832 [doi]
AID - S0042-6822(99)99832-X [pii]
PST - ppublish
SO  - Virology. 1999 Aug 1;260(2):316-28. doi: 10.1006/viro.1999.9832.