PMID- 10415339
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 235
IP  - 1-2
DP  - 1999 Jul 22
TI  - Cloning and gene structure of rat phosphatidylcholine transfer protein, Pctp.
PG  - 111-20
AB  - Phosphatidylcholine transfer protein (PC-TP) is a cytosolic lipid transfer
      protein that promotes intermembrane transfer of phosphatidylcholines but no other
      phospholipids. Although its physiological function remains unknown,
      phosphatidylcholine transfer protein is enriched in liver and evidence from model
      systems suggests a role in hepatocellular selection and transport of biliary
      phospholipids. To facilitate in vivo studies, a cDNA encoding rat PC-TP was
      cloned by library screening and 5'-rapid amplification of cDNA ends. Genomic
      cloning demonstrated the rat Pctp gene spans 10. 8kb and is comprised of six
      exons. The putative transcription initiation site was identified 50bp upstream of
      the translation initiation site. Nucleotide sequence analysis of the 5'-flanking 
      region revealed a CAAT- but no TATA-box. Transient transfection of a series of
      5'-deleted Pctp-promoter-firefly luciferase constructs into Reuber H35 rat
      hepatoma cells, which express Pctp mRNA, and Gunn rat fibroblasts, which do not, 
      suggest that cis-acting elements in a 637bp promoter region contribute to
      enhanced expression of PC-TP in liver.
FAU - Wu, M K
AU  - Wu MK
AD  - Marion Bessin Liver Research Center, Department of Medicine, Albert Einstein
      College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461, USA.
FAU - Boylan, M O
AU  - Boylan MO
FAU - Cohen, D E
AU  - Cohen DE
LA  - eng
SI  - GENBANK/AF040261
SI  - GENBANK/AF040262
SI  - GENBANK/AF040263
SI  - GENBANK/AF040264
SI  - GENBANK/AF040265
SI  - GENBANK/AF040266
SI  - GENBANK/AF040267
GR  - DK48873/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Androgen-Binding Protein)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Phospholipid Transfer Proteins)
RN  - 0 (Prostatein)
RN  - 0 (Psbpc1 protein, rat)
RN  - 0 (Psbpc2 protein, rat)
RN  - 0 (RNA, Messenger)
RN  - 0 (Scgb2a2 protein, rat)
RN  - 0 (Secretoglobins)
RN  - 9060-09-7 (Uteroglobin)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Androgen-Binding Protein
MH  - Animals
MH  - Base Sequence
MH  - Carrier Proteins/chemistry/*genetics/metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Exons/genetics
MH  - Gene Expression Regulation
MH  - Introns/genetics
MH  - Liver/metabolism
MH  - Molecular Sequence Data
MH  - Phospholipid Transfer Proteins
MH  - Promoter Regions, Genetic/genetics
MH  - Prostatein
MH  - RNA, Messenger/analysis/genetics
MH  - Rats
MH  - Response Elements/genetics
MH  - Secretoglobins
MH  - Sequence Deletion
MH  - Transfection
MH  - Uteroglobin
EDAT- 1999/07/23 00:00
MHDA- 1999/07/23 00:01
CRDT- 1999/07/23 00:00
PHST- 1999/07/23 00:00 [pubmed]
PHST- 1999/07/23 00:01 [medline]
PHST- 1999/07/23 00:00 [entrez]
AID - S0378-1119(99)00204-8 [pii]
AID - 10.1016/s0378-1119(99)00204-8 [doi]
PST - ppublish
SO  - Gene. 1999 Jul 22;235(1-2):111-20. doi: 10.1016/s0378-1119(99)00204-8.