PMID- 10415332
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20191210
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 235
IP  - 1-2
DP  - 1999 Jul 22
TI  - Rat endozepine-like peptide (ELP): cDNA cloning, genomic organization and
      tissue-specific expression.
PG  - 51-7
AB  - A cDNA encoding the rat homolog of the previously characterized murine
      endozepine-like peptide (ELP) was isolated by a PCR cloning strategy. Sequence
      comparison with the murine cDNA sequence revealed a conservation of the ELP
      primary structure between both rodent species with minor amino acid exchanges. We
      investigated the genomic organization of the rat ELP gene by genomic PCR. This
      indicated the presence of a single short intron of 451bp interrupting the 5'
      untranslated region. Tissue-dependent ELP expression was determined by Northern
      hybridization and semiquantitative RT-PCR. Northern hybridization showed an ELP
      specific transcript in both the male and the female gonad, but the level of
      ovarian ELP transcription was considerably lower than in the testis. RT-PCR
      analysis demonstrated a low and varying level of ELP background expression in all
      examined tissues. In contrast to the closely related ACBP gene, ELP shows a
      different genomic organization and a more regulated expression pattern, and may
      exert a specific function as a gonadal acyl-CoA pool former and transporter.
FAU - Pusch, W
AU  - Pusch W
AD  - Institute of Anatomy, University of Hamburg, Martinistrasse 52, 20246, Hamburg,
      Germany.
FAU - Jahner, D
AU  - Jahner D
FAU - Spiess, A N
AU  - Spiess AN
FAU - Ivell, R
AU  - Ivell R
LA  - eng
SI  - GENBANK/AF128092
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Diazepam Binding Inhibitor)
RN  - 0 (Peptides)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (endozepine-like peptide ELP)
SB  - IM
MH  - 5' Untranslated Regions/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Carrier Proteins/chemistry/genetics/physiology
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Diazepam Binding Inhibitor
MH  - Female
MH  - *Gene Expression
MH  - *Genome
MH  - Introns/genetics
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Ovary/metabolism
MH  - Peptides
MH  - Proteins/chemistry/*genetics/physiology
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Testis/metabolism
EDAT- 1999/07/23 00:00
MHDA- 1999/07/23 00:01
CRDT- 1999/07/23 00:00
PHST- 1999/07/23 00:00 [pubmed]
PHST- 1999/07/23 00:01 [medline]
PHST- 1999/07/23 00:00 [entrez]
AID - S0378-1119(99)00216-4 [pii]
AID - 10.1016/s0378-1119(99)00216-4 [doi]
PST - ppublish
SO  - Gene. 1999 Jul 22;235(1-2):51-7. doi: 10.1016/s0378-1119(99)00216-4.