PMID- 10415329
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 235
IP  - 1-2
DP  - 1999 Jul 22
TI  - Cloning, gene expression, and characterization of CP27, a novel gene in mouse
      embryogenesis.
PG  - 19-30
AB  - We report the full-length sequencing, tissue-specific expression, and
      immunolocalization of cp27, a novel gene in mouse embryogenesis. The cp27 gene
      was isolated and cloned from a mouse E11 lambdagt11 library using a peptide
      antibody that recognized a distinct expression pattern in mouse craniofacial
      development. The cp27 gene contains an open reading frame of 295 amino acids
      corresponding to a predicted molecular mass of 33kDa. On Western blots, a
      polyclonal antibody against CP27 detected a single epitope at 27kDa. The putative
      CP27 protein has an isoelectric point of 4.75 and features a distinct
      helix-loop-helix structure according to prediction algorithms. We have cloned the
      human cp27 gene and mapped it to a locus on the human chromosome 16 which is in
      proximity to several loci associated with inherited craniofacial diseases such as
      fanconi anemia type A. Northern blot analysis of RNA from multiple mouse tissues 
      demonstrated high levels of expression in developing mouse teeth, heart, lung,
      and liver of a single transcript of approx. 1. 8kbp. In situ hybridization using 
      a radioactive RNA probe resulted in distinct signals in the developing
      neuroepithelium, cerebellum, heart, lung, liver, teeth, salivary glands, and
      periosteum of developing bones. Immunohistochemical staining of developing mouse 
      tissues detected epitopes specific for CP27 in the mesenchyme surrounding the
      primary brain vesicles, in basement membranes, in the periosteum, in salivary
      glands, and in the stellate reticulum of teeth. Thus, CP27 represents a unique
      gene product involved in mouse embryogenesis.
FAU - Diekwisch, T G
AU  - Diekwisch TG
AD  - Center for Craniofacial Molecular Biology, University of Southern California, Los
      Angeles, CA 90033, USA. tomdkw@tambcd.edu
FAU - Marches, F
AU  - Marches F
FAU - Williams, A
AU  - Williams A
FAU - Luan, X
AU  - Luan X
LA  - eng
SI  - GENBANK/Y08219
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (CFDP1 protein, human)
RN  - 0 (Cfdp1 protein, mouse)
RN  - 0 (Epitopes)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Central Nervous System/embryology/metabolism
MH  - Chromosomes, Human, Pair 16/genetics
MH  - Cloning, Molecular
MH  - Embryo, Mammalian/*metabolism
MH  - Embryonic and Fetal Development
MH  - Epitopes/immunology
MH  - Extracellular Matrix Proteins
MH  - *Gene Expression Regulation, Developmental
MH  - Helix-Loop-Helix Motifs
MH  - Humans
MH  - Isoelectric Point
MH  - Mesoderm/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phosphoproteins/chemistry/genetics
MH  - *Physical Chromosome Mapping
MH  - Protein Structure, Secondary
MH  - Proteins/analysis/chemistry/*genetics/immunology
MH  - RNA, Messenger/genetics/metabolism
MH  - Recombinant Fusion Proteins/analysis/immunology
MH  - Sequence Homology, Amino Acid
MH  - Tooth/embryology/metabolism
EDAT- 1999/07/23 00:00
MHDA- 1999/07/23 00:01
CRDT- 1999/07/23 00:00
PHST- 1999/07/23 00:00 [pubmed]
PHST- 1999/07/23 00:01 [medline]
PHST- 1999/07/23 00:00 [entrez]
AID - S0378-1119(99)00220-6 [pii]
AID - 10.1016/s0378-1119(99)00220-6 [doi]
PST - ppublish
SO  - Gene. 1999 Jul 22;235(1-2):19-30. doi: 10.1016/s0378-1119(99)00220-6.