PMID- 10415121
OWN - NLM
STAT- MEDLINE
DCOM- 19990908
LR  - 20141120
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 368
IP  - 1
DP  - 1999 Aug 1
TI  - In vitro processing of the human alkyl-dihydroxyacetonephosphate synthase
      precursor.
PG  - 139-46
AB  - Alkyl-dihydroxyacetonephosphate synthase, a peroxisomal enzyme involved in the
      biosynthesis of ether phospholipids, is synthesized with a cleavable N-terminal
      presequence containing the peroxisomal targeting signal type 2. The human
      alkyl-dihydroxyacetonephosphate synthase precursor produced in vitro or expressed
      in Escherichia coli could be processed to a lower molecular weight protein by
      incubation at 37 degrees C with a guinea pig liver fraction, enriched in
      mitochondria, lysosomes, and peroxisomes. This lower molecular weight protein was
      identified as the mature human alkyl-dihydroxyacetonephosphate synthase by
      radiosequencing, indicating that the processing protease is present in this
      organellar fraction. Characterization of the processing protease indicated that
      it is a cysteine protease with a pH optimum of 6.5. Furthermore, it was
      demonstrated that exogenously added pre-alkyl-dihydroxyacetonephosphate synthase 
      was imported and processed in purified peroxisomes in vitro. Processing of
      alkyl-dihydroxyacetonephosphate synthase did not increase the activity of the
      enzyme. This indicates that the presence of the presequence does not affect the
      activity of the enzyme.
CI  - Copyright 1999 Academic Press.
FAU - Biermann, J
AU  - Biermann J
AD  - Institute for Biomembranes, Utrecht University, Utrecht, The Netherlands.
FAU - van den Bosch, H
AU  - van den Bosch H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Enzyme Precursors)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.5.- (Alkyl and Aryl Transferases)
RN  - EC 2.5.1.26 (alkylglycerone-phosphate synthase)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
SB  - IM
MH  - Alkyl and Aryl Transferases/chemistry/genetics/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cysteine Endopeptidases/metabolism
MH  - Enzyme Activation
MH  - Enzyme Precursors/chemistry/genetics/*metabolism
MH  - Guinea Pigs
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Liver/metabolism
MH  - Microbodies/enzymology
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Protein Processing, Post-Translational
MH  - Recombinant Proteins/genetics/metabolism
MH  - Sequence Homology, Amino Acid
EDAT- 1999/07/23 00:00
MHDA- 1999/07/23 00:01
CRDT- 1999/07/23 00:00
PHST- 1999/07/23 00:00 [pubmed]
PHST- 1999/07/23 00:01 [medline]
PHST- 1999/07/23 00:00 [entrez]
AID - 10.1006/abbi.1999.1281 [doi]
AID - S0003-9861(99)91281-X [pii]
PST - ppublish
SO  - Arch Biochem Biophys. 1999 Aug 1;368(1):139-46. doi: 10.1006/abbi.1999.1281.