PMID- 10413680
OWN - NLM
STAT- MEDLINE
DCOM- 19991028
LR  - 20081121
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 112 ( Pt 16)
DP  - 1999 Aug
TI  - mAKAP: an A-kinase anchoring protein targeted to the nuclear membrane of
      differentiated myocytes.
PG  - 2725-36
AB  - The compartmentalization of second messenger-activated protein kinases
      contributes to the fidelity of hormone-mediated signal transduction events. For
      example, the cAMP-dependent protein kinase is tethered at specific intracellular 
      locations through association with A-kinase anchoring proteins (AKAPs). We now
      report the cloning of mAKAP, an anchoring protein found predominantly in heart,
      skeletal muscle and brain, and whose expression is induced in neonatal
      ventriculocytes by treatment with hypertrophic stimuli. mAKAP is targeted to the 
      nuclear membrane of differentiated myocytes. Analysis of mAKAP-green fluorescent 
      protein (GFP) fusion constructs revealed that nuclear membrane targeting is
      conferred by two regions of the protein, between residues 772-915 and 915-1065,
      which contain spectrin-like repeat sequences. Heterologous expression of the
      mAKAP targeting sequences displaced the endogenous anchoring protein from the
      nuclear membrane, demonstrating that mAKAP targeting is saturable. Collectively, 
      these data suggest that a domain containing spectrin-like repeats mediates
      targeting of the anchoring protein mAKAP and the cAMP-dependent protein kinase
      holoenzyme to the nuclear membrane in response to differentiation signals.
FAU - Kapiloff, M S
AU  - Kapiloff MS
AD  - Howard Hughes Medical Institute, L-474, Vollum Institute, Portland OR 97201-3098,
      USA.
FAU - Schillace, R V
AU  - Schillace RV
FAU - Westphal, A M
AU  - Westphal AM
FAU - Scott, J D
AU  - Scott JD
LA  - eng
GR  - DK44239/DK/NIDDK NIH HHS/United States
GR  - K08 CA69137/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (A Kinase Anchor Proteins)
RN  - 0 (AKAP6 protein, human)
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Akap6 protein, rat)
RN  - 0 (Antibodies)
RN  - 0 (Carrier Proteins)
RN  - 0 (Peptide Fragments)
RN  - 12634-43-4 (Spectrin)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - A Kinase Anchor Proteins
MH  - *Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Animals, Newborn
MH  - Antibodies
MH  - Carrier Proteins/chemistry/genetics/*metabolism
MH  - Cell Differentiation/physiology
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - Cyclic AMP-Dependent Protein Kinases/*analysis
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Regulation, Enzymologic
MH  - Heart Ventricles/cytology
MH  - Humans
MH  - Microscopy, Confocal
MH  - Molecular Sequence Data
MH  - Muscle Fibers, Skeletal/*chemistry/cytology/enzymology
MH  - Mutagenesis/physiology
MH  - Myocardium/*chemistry/cytology/enzymology
MH  - Nuclear Envelope/*chemistry/enzymology
MH  - Peptide Fragments/analysis/immunology
MH  - Protein Binding/physiology
MH  - Protein Structure, Tertiary
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Spectrin/analysis/chemistry/genetics
EDAT- 1999/07/22 00:00
MHDA- 1999/07/22 00:01
CRDT- 1999/07/22 00:00
PHST- 1999/07/22 00:00 [pubmed]
PHST- 1999/07/22 00:01 [medline]
PHST- 1999/07/22 00:00 [entrez]
PST - ppublish
SO  - J Cell Sci. 1999 Aug;112 ( Pt 16):2725-36.