PMID- 10413677
OWN - NLM
STAT- MEDLINE
DCOM- 19991028
LR  - 20131121
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 112 ( Pt 16)
DP  - 1999 Aug
TI  - Nucleolar localization of murine nuclear DNA helicase II (RNA helicase A).
PG  - 2693-703
AB  - Nuclear DNA helicase II (NDH II) is a highly conserved member of the DEXH
      superfamily of eukaryotic helicases, whose physiological role is still unclear.
      To explore the function of NDH II, we studied the intracellular distribution of
      NDH II of different mammalian species by immunofluorescence and compared these
      findings with the known role of the Drosophila homologue MLE that is involved in 
      sex-specific gene dosage compensation. NDH II displayed an apparent nucleolar
      localization in murine cells, whereas in cells from all other mammalian species
      examined so far the protein was confined to the nucleoplasm and apparently
      excluded from the nucleoli. The nucleolar localization of mouse NDH II strongly
      suggests a role in ribosomal RNA biosynthesis. Immunoelectron microscopic studies
      revealed that the mouse NDH II was found at the dense fibrillar components of the
      nucleoli, and a significant percentage of NDH II molecules colocalized with the
      RNA polymerase I (Pol I) transcription factor UBF (upstream binding factor).
      Additionally, the nucleolar localization of NDH II coincided with a preferential 
      immunolabeling pattern of nascent transcripts with bromouridine (BrUMP).
      Furthermore, mouse NDH II redistributed in mitosis in a manner highly correlated 
      with Pol I activity. Conditions leading to the inhibition of Pol I activity in
      the interphase decreased the amount of NDH II in the nucleoli that diffused into 
      the nucleoplasm and the cytosol. Contrary to the effect of inhibiting rRNA
      synthesis, treatment of mouse cells with the translation inhibitor cycloheximide 
      did not compromise the nucleolar localization of murine NDH II.
FAU - Zhang, S
AU  - Zhang S
AD  - Department of Biochemistry and Department of Electron Microscopy and Molecular
      Cytology, Institute for Molecular Biotechnology, Beutenbergstrasse 11, D-07745
      Jena, Germany.
FAU - Herrmann, C
AU  - Herrmann C
FAU - Grosse, F
AU  - Grosse F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Dhx9 protein, mouse)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (RNA, Ribosomal)
RN  - 3398-50-3 (5-bromouridine triphosphate)
RN  - 98600C0908 (Cycloheximide)
RN  - EC 3.6.- (Acid Anhydride Hydrolases)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.1.15 (Nucleoside-Triphosphatase)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
RN  - EC 3.6.4.13 (RNA Helicases)
RN  - UT0S826Z60 (Uridine Triphosphate)
SB  - IM
MH  - 3T3 Cells
MH  - Acid Anhydride Hydrolases/analysis/genetics
MH  - Adenosine Triphosphatases/analysis/*genetics
MH  - Animals
MH  - Cell Nucleolus/*enzymology/genetics/ultrastructure
MH  - Cycloheximide/pharmacology
MH  - DEAD-box RNA Helicases
MH  - DNA Helicases/analysis/*genetics
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Regulation, Enzymologic/drug effects/physiology
MH  - HeLa Cells
MH  - Humans
MH  - Mice
MH  - Microscopy, Immunoelectron
MH  - Mitosis/physiology
MH  - Molecular Sequence Data
MH  - Nucleoside-Triphosphatase
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - RNA Helicases/analysis/genetics
MH  - RNA, Ribosomal/biosynthesis/genetics
MH  - Rabbits
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Transcription, Genetic/drug effects/physiology
MH  - Uridine Triphosphate/analogs & derivatives/pharmacology
EDAT- 1999/07/22 00:00
MHDA- 1999/07/22 00:01
CRDT- 1999/07/22 00:00
PHST- 1999/07/22 00:00 [pubmed]
PHST- 1999/07/22 00:01 [medline]
PHST- 1999/07/22 00:00 [entrez]
PST - ppublish
SO  - J Cell Sci. 1999 Aug;112 ( Pt 16):2693-703.