PMID- 10413673
OWN - NLM
STAT- MEDLINE
DCOM- 19991028
LR  - 20091119
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 112 ( Pt 16)
DP  - 1999 Aug
TI  - The casein kinase Ialpha isoform is both physically positioned and functionally
      competent to regulate multiple events of mRNA metabolism.
PG  - 2647-56
AB  - Casein kinase I is a highly conserved family of serine/threonine protein kinases 
      present in every organism tested from yeast to humans. To date, little is known
      about the function of the higher eukaryotic isoforms in this family. The CKI
      isoforms in Saccharomyces cerevisiae, however, have been genetically linked to
      the regulation of DNA repair, cell cycle progression and cytokinesis. It has also
      been established that the nuclear localization of two of these isoforms is
      essential for their function. The work presented here demonstrates that the
      higher eukaryotic CKIalpha isoform is also present within nuclei of certain
      established cell lines and associated with discrete nuclear structures. The
      nature of its nuclear localization was characterized. In this regard, CKIalpha
      was shown to colocalize with factors involved in pre-mRNA splicing at nuclear
      speckles and that its association with these structures exhibited several
      biochemical properties in common with known splicing factors. The kinase was also
      shown to be associated with a complex that contained certain splicing factors.
      Finally, in vitro, CKIalpha was shown to be capable of phosphorylating particular
      splicing factors within a region rich in serine/arginine dipeptide repeat motifs 
      suggesting that it has both the opportunity and the capacity to regulate one or
      more steps of mRNA metabolism.
FAU - Gross, S D
AU  - Gross SD
AD  - Department of Pharmacology, University of Wisconsin Medical School, Madison, WI, 
      USA.
FAU - Loijens, J C
AU  - Loijens JC
FAU - Anderson, R A
AU  - Anderson RA
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (Casein Kinases)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 3.1.- (Deoxyribonucleases)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Animals
MH  - Casein Kinases
MH  - Cell Nucleus/enzymology
MH  - Deoxyribonucleases/pharmacology
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Regulation, Enzymologic
MH  - HeLa Cells
MH  - Humans
MH  - Isoenzymes/analysis/*metabolism
MH  - Kidney/cytology
MH  - Phosphorylation
MH  - Protein Kinases/analysis/*metabolism
MH  - Protein Structure, Tertiary
MH  - Protein-Serine-Threonine Kinases/analysis/chemistry/metabolism
MH  - RNA Processing, Post-Transcriptional/physiology
MH  - RNA Splicing/physiology
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Ribonucleases/pharmacology
MH  - Transcription, Genetic/physiology
EDAT- 1999/07/22 00:00
MHDA- 1999/07/22 00:01
CRDT- 1999/07/22 00:00
PHST- 1999/07/22 00:00 [pubmed]
PHST- 1999/07/22 00:01 [medline]
PHST- 1999/07/22 00:00 [entrez]
PST - ppublish
SO  - J Cell Sci. 1999 Aug;112 ( Pt 16):2647-56.