PMID- 10413512
OWN - NLM
STAT- MEDLINE
DCOM- 19990915
LR  - 20131121
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 38
IP  - 29
DP  - 1999 Jul 20
TI  - Histidine-13 is a crucial residue in the zinc ion-induced aggregation of the A
      beta peptide of Alzheimer's disease.
PG  - 9373-8
AB  - Metal ions such as Zn(2+) and Cu(2+) have been implicated in both the aggregation
      and neurotoxicity of the beta-amyloid (Abeta) peptide that is present in the
      brains of Alzheimer's sufferers. Zinc ions in particular have been shown to
      induce rapid aggregation of Abeta. Rat Abeta binds zinc ions much less avidly
      than human Abeta, and rats do not form cerebral Abeta amyloid. Rat Abeta differs 
      from human Abeta by the substitution of Gly for Arg, Phe for Tyr, and Arg for His
      at positions 5, 10, and 13, respectively. Through the use of synthetic peptides
      corresponding to the first 28 residues of human Abeta, rat Abeta, and
      single-residue variations, we use circular dichroism spectroscopy, sedimentation 
      assays, and immobilized metal ion affinity chromatography to show that the
      substitution of Arg for His-13 is responsible for the different Zn(2+)-induced
      aggregation behavior of rat and human Abeta. The coordination of Zn(2+) to
      histidine-13 is critical to the zinc ion induced aggregation of Abeta.
FAU - Liu, S T
AU  - Liu ST
AD  - School of Chemistry, Department of Biochemistry and Molecular Biology, The
      University of Melbourne, Parkville, Victoria, Australia.
FAU - Howlett, G
AU  - Howlett G
FAU - Barrow, C J
AU  - Barrow CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Cations, Divalent)
RN  - 0 (Chelating Agents)
RN  - 0 (Peptide Fragments)
RN  - 0 (Solutions)
RN  - 0 (amyloid beta-protein (1-28))
RN  - 4QD397987E (Histidine)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Alzheimer Disease/*metabolism
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - Amyloid beta-Peptides/chemistry/*metabolism
MH  - Animals
MH  - Cations, Divalent/chemistry/metabolism
MH  - Chelating Agents/chemistry/metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Circular Dichroism
MH  - Dose-Response Relationship, Drug
MH  - Histidine/chemistry/*metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Peptide Fragments/chemistry/metabolism
MH  - Protein Structure, Secondary
MH  - Rats
MH  - Solutions
MH  - Zinc/chemistry/*metabolism
EDAT- 1999/07/22 00:00
MHDA- 1999/07/22 00:01
CRDT- 1999/07/22 00:00
PHST- 1999/07/22 00:00 [pubmed]
PHST- 1999/07/22 00:01 [medline]
PHST- 1999/07/22 00:00 [entrez]
AID - 10.1021/bi990205o [doi]
AID - bi990205o [pii]
PST - ppublish
SO  - Biochemistry. 1999 Jul 20;38(29):9373-8. doi: 10.1021/bi990205o.