PMID- 10413453
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20061115
IS  - 0022-3034 (Print)
IS  - 0022-3034 (Linking)
VI  - 40
IP  - 2
DP  - 1999 Aug
TI  - Down-regulation of striatin, a neuronal calmodulin-binding protein, impairs rat
      locomotor activity.
PG  - 234-43
AB  - Striatin, an intraneuronal, calmodulin-binding protein addressed to dendrites and
      spines, is expressed in the motor system, particularly the striatum and
      motoneurons. Striatin contains a high number of domains mediating protein-protein
      interactions, suggesting a role within a dendritic Ca(2+)-signaling pathway.
      Here, we explored the hypothesis of a direct role of striatin in the motor
      control of behaving rats, by using an antisense strategy based on
      oligodeoxynucleotides (ODN). Rats were treated by intracerebroventricular
      infusion of a striatin antisense ODN (A-ODN) or mismatch ODN (M-ODN) delivered by
      osmotic pumps over 6 days. A significant decrease in the nocturnal locomotor
      activity of A-ODN-treated rats was observed after 5 days of treatment.
      Hypomotricity was correlated with a 60% decrease in striatin content of the
      striata of A-ODN-treated rats sacrificed on day 6. Striatin thus plays a role in 
      the control of motor function. To approach the cellular mechanisms in which
      striatin is involved, striatin down-regulation was studied in a comparatively
      simpler model: purified rat spinal motoneurons which retain their polarity in
      culture. Treatment of cells by the striatin A-ODN resulted in the impairement of 
      the growth of dendrites but not axon. The decrease in dendritic growth paralleled
      the loss of striatin. This model allows analysis of the molecular basis of
      striatin function in the dynamic changes occurring in growing dendrites, and
      offers clues to unravel its function within spines.
CI  - Copyright 1999 John Wiley & Sons, Inc.
FAU - Bartoli, M
AU  - Bartoli M
AD  - Unite de Neurocybernetique Cellulaire, CNRS UPR 9041, 31 chemin Joseph Aiguier,
      13402 Marseille, France.
FAU - Ternaux, J P
AU  - Ternaux JP
FAU - Forni, C
AU  - Forni C
FAU - Portalier, P
AU  - Portalier P
FAU - Salin, P
AU  - Salin P
FAU - Amalric, M
AU  - Amalric M
FAU - Monneron, A
AU  - Monneron A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurobiol
JT  - Journal of neurobiology
JID - 0213640
RN  - 0 (Calmodulin)
RN  - 0 (Calmodulin-Binding Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Strn protein, rat)
SB  - IM
MH  - Animals
MH  - Calmodulin/metabolism
MH  - Calmodulin-Binding Proteins/biosynthesis/deficiency/genetics/*physiology
MH  - Cells, Cultured
MH  - Corpus Striatum/drug effects/metabolism/*physiopathology
MH  - Dendrites/ultrastructure
MH  - Depression, Chemical
MH  - Gene Expression Regulation/*drug effects
MH  - Injections, Intraventricular
MH  - Locomotion/*physiology
MH  - Male
MH  - Membrane Proteins/biosynthesis/deficiency/genetics/*physiology
MH  - Motor Neurons/drug effects/pathology
MH  - Movement Disorders/genetics/*physiopathology
MH  - Nerve Tissue Proteins/biosynthesis/deficiency/genetics/*physiology
MH  - Oligonucleotides, Antisense/administration & dosage/pharmacology/toxicity
MH  - Rats
MH  - Rats, Wistar
MH  - Spinal Cord/cytology
EDAT- 1999/07/21 00:00
MHDA- 1999/07/21 00:01
CRDT- 1999/07/21 00:00
PHST- 1999/07/21 00:00 [pubmed]
PHST- 1999/07/21 00:01 [medline]
PHST- 1999/07/21 00:00 [entrez]
AID - 10.1002/(SICI)1097-4695(199908)40:2<234::AID-NEU9>3.0.CO;2-T [pii]
PST - ppublish
SO  - J Neurobiol. 1999 Aug;40(2):234-43.