PMID- 10413105
OWN - NLM
STAT- MEDLINE
DCOM- 19990809
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 454
IP  - 1-2
DP  - 1999 Jul 2
TI  - Interaction of soluble and surface-bound heparin binding growth-associated
      molecule with heparin.
PG  - 105-8
AB  - The interaction of heparin with heparin binding growth-associated molecule
      (HB-GAM) was studied using isothermal titration calorimetry (ITC) and surface
      plasmon resonance (SPR). ITC studies showed that, in solution, heparin bound
      HB-GAM with a deltaH of -30 kcal/mole corresponding to a dissociation constant
      (Kd) of 460 nM. The stoichiometry of interaction was 3 moles of HB-GAM per mole
      of heparin, corresponding to a minimum heparin binding site for HB-GAM of 12-16
      saccharide residues. Kinetic measurements of heparin interaction with HB-GAM made
      by SPR afforded a Kd of 4 nM, suggesting considerably tighter binding when HB-GAM
      was immobilized on a surface. Affinity chromatography of a sized mixture of
      heparin oligosaccharides, having a degree of polymerization (dp) of > 14
      saccharide units, on HB-GAM-Sepharose demonstrated that oligosaccharides having
      more than 18 saccharide residues showed the tightest interaction.
FAU - Fath, M
AU  - Fath M
AD  - Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City
      52242, USA.
FAU - VanderNoot, V
AU  - VanderNoot V
FAU - Kilpelainen, I
AU  - Kilpelainen I
FAU - Kinnunen, T
AU  - Kinnunen T
FAU - Rauvala, H
AU  - Rauvala H
FAU - Linhardt, R J
AU  - Linhardt RJ
LA  - eng
GR  - GM38060/GM/NIGMS NIH HHS/United States
GR  - HL52622/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytokines)
RN  - 134034-50-7 (pleiotrophin)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Calorimetry
MH  - Carrier Proteins/*metabolism
MH  - Chromatography, Affinity
MH  - Cytokines/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Heparin/*metabolism
MH  - Kinetics
MH  - Swine
MH  - Thermodynamics
MH  - Time Factors
EDAT- 1999/07/21 00:00
MHDA- 1999/07/21 00:01
CRDT- 1999/07/21 00:00
PHST- 1999/07/21 00:00 [pubmed]
PHST- 1999/07/21 00:01 [medline]
PHST- 1999/07/21 00:00 [entrez]
AID - S0014-5793(99)00785-1 [pii]
AID - 10.1016/s0014-5793(99)00785-1 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Jul 2;454(1-2):105-8. doi: 10.1016/s0014-5793(99)00785-1.