PMID- 10413101
OWN - NLM
STAT- MEDLINE
DCOM- 19990809
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 454
IP  - 1-2
DP  - 1999 Jul 2
TI  - Arfaptin 1 forms a complex with ADP-ribosylation factor and inhibits
      phospholipase D.
PG  - 85-9
AB  - ADP-ribosylation factors (ARFs) regulate coatomer assembly on the Golgi as well
      as recruitment of clathrin adapter proteins and are therefore involved in vesicle
      budding from the Golgi and vesicular transport. They are also regulators of
      phospholipase D (PLD) activity. Arfaptin 1 is an ARF binding protein that
      inhibits PLD activation, vesicular trafficking and secretion. In the present
      report, we show that arfaptin 1 interacts with 'high speed' membranes
      independently of ARF. However, addition of myristoylated ARF3 (myrARF3) increases
      the association of arfaptin 1 with the membranes, suggesting that arfaptin 1 and 
      ARF form a complex on the Golgi. Utilizing several deletion mutants of arfaptin 1
      it is shown that the association of arfaptin 1 with myrARF3 is mediated via two
      binding sites on arfaptin 1. These two domains are needed for arfaptin 1
      inhibition of PLD activation by myrARF3 in vitro.
FAU - Williger, B T
AU  - Williger BT
AD  - Howard Hughes Medical Institute and Department of Molecular Physiology and
      Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232-0295,
      USA.
FAU - Provost, J J
AU  - Provost JJ
FAU - Ho, W T
AU  - Ho WT
FAU - Milstine, J
AU  - Milstine J
FAU - Exton, J H
AU  - Exton JH
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (ARFIP1 protein, human)
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 3.1.4.4 (Phospholipase D)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (ADP-Ribosylation Factors)
SB  - IM
MH  - ADP-Ribosylation Factors
MH  - *Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Carrier Proteins/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Escherichia coli/metabolism
MH  - GTP-Binding Proteins/*metabolism
MH  - Glutathione Transferase/metabolism
MH  - Golgi Apparatus/drug effects
MH  - Liver/metabolism
MH  - Phospholipase D/*antagonists & inhibitors
MH  - Rats
MH  - Recombinant Fusion Proteins/pharmacology
MH  - Tumor Cells, Cultured
EDAT- 1999/07/21 00:00
MHDA- 1999/07/21 00:01
CRDT- 1999/07/21 00:00
PHST- 1999/07/21 00:00 [pubmed]
PHST- 1999/07/21 00:01 [medline]
PHST- 1999/07/21 00:00 [entrez]
AID - S0014-5793(99)00771-1 [pii]
AID - 10.1016/s0014-5793(99)00771-1 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Jul 2;454(1-2):85-9. doi: 10.1016/s0014-5793(99)00771-1.