PMID- 10412982
OWN - NLM
STAT- MEDLINE
DCOM- 19990809
LR  - 20161019
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 98
IP  - 1
DP  - 1999 Jul 9
TI  - Endomembrane trafficking of ras: the CAAX motif targets proteins to the ER and
      Golgi.
PG  - 69-80
AB  - We show that Nras is transiently localized in the Golgi prior to the plasma
      membrane (PM). Moreover, green fluorescent protein (GFP)-tagged Nras illuminated 
      motile, peri-Golgi vesicles, and prolonged BFA treatment blocked PM expression.
      GFP-Hras colocalized with GFP-Nras, but GFP-Kras4B revealed less Golgi and no
      vesicular fluorescence. Whereas a secondary membrane targeting signal was
      required for PM expression, the CAAX motif alone was necessary and sufficient to 
      target proteins to the endomembrane where they were methylated, a modification
      required for efficient membrane association. Thus, prenylated CAAX proteins do
      not associate directly with the PM but instead associate with the endomembrane
      and are subsequently transported to the PM, a process that requires a secondary
      targeting motif.
FAU - Choy, E
AU  - Choy E
AD  - Department of Medicine, New York University School of Medicine, New York 10016,
      USA.
FAU - Chiu, V K
AU  - Chiu VK
FAU - Silletti, J
AU  - Silletti J
FAU - Feoktistov, M
AU  - Feoktistov M
FAU - Morimoto, T
AU  - Morimoto T
FAU - Michaelson, D
AU  - Michaelson D
FAU - Ivanov, I E
AU  - Ivanov IE
FAU - Philips, M R
AU  - Philips MR
LA  - eng
GR  - R01 AI036224/AI/NIAID NIH HHS/United States
GR  - AI36224/AI/NIAID NIH HHS/United States
GR  - GM55279/GM/NIGMS NIH HHS/United States
GR  - M01 RR00096/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Luminescent Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 3.6.5.2 (ras Proteins)
SB  - IM
CIN - Cell. 1999 Jul 9;98(1):9-12. PMID: 10412976
MH  - Amino Acid Sequence
MH  - Animals
MH  - CHO Cells
MH  - COS Cells
MH  - Cell Line
MH  - Cell Membrane/*metabolism
MH  - Cricetinae
MH  - Dogs
MH  - Endoplasmic Reticulum/*metabolism
MH  - Golgi Apparatus/*metabolism
MH  - Green Fluorescent Proteins
MH  - Kidney
MH  - Kinetics
MH  - Luminescent Proteins/genetics/metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Transfection
MH  - ras Proteins/chemistry/genetics/*metabolism
EDAT- 1999/07/21 00:00
MHDA- 1999/07/21 00:01
CRDT- 1999/07/21 00:00
PHST- 1999/07/21 00:00 [pubmed]
PHST- 1999/07/21 00:01 [medline]
PHST- 1999/07/21 00:00 [entrez]
AID - S0092-8674(00)80607-8 [pii]
AID - 10.1016/S0092-8674(00)80607-8 [doi]
PST - ppublish
SO  - Cell. 1999 Jul 9;98(1):69-80. doi: 10.1016/S0092-8674(00)80607-8.