PMID- 10411930
OWN - NLM
STAT- MEDLINE
DCOM- 19990823
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 15
DP  - 1999 Jul 20
TI  - Elevated retinoic acid receptor beta(4) protein in human breast tumor cells with 
      nuclear and cytoplasmic localization.
PG  - 8651-6
AB  - The transcription factor retinoic acid receptor beta(2) (RARbeta(2)) is a potent 
      inhibitor of breast cancer cells in vitro, and studies suggest that RARbeta
      expression is lost in primary breast cancer. Although RARbeta(2) is selectively
      down-regulated at the mRNA level in breast tumor cells, we show that expression
      of an RARbeta protein is elevated in five of five breast tumor cell lines
      relative to normal human mammary epithelial cells. Subsequent analysis identified
      this protein as the translation product of the human RARbeta(4) transcript.
      Unlike the previously characterized mouse RARbeta(4) isoform, the human
      RARbeta(4) retains only half of a DNA-binding domain and lacks a
      ligand-independent transactivation domain at its N terminus. The RARbeta(4)
      protein localizes to the cytoplasm and to subnuclear compartments that resemble
      nuclear bodies. The structure and preliminary characterizations of human
      RARbeta(4), coupled with the observation that its expression is greatly elevated 
      in breast tumor cell lines, support the hypothesis that RARbeta(4) functions as a
      dominant-negative repressor of RAR-mediated growth suppression.
FAU - Sommer, K M
AU  - Sommer KM
AD  - Department of Pathology, University of Washington, Seattle, WA 98195, USA.
FAU - Chen, L I
AU  - Chen LI
FAU - Treuting, P M
AU  - Treuting PM
FAU - Smith, L T
AU  - Smith LT
FAU - Swisshelm, K
AU  - Swisshelm K
LA  - eng
SI  - GENBANK/AF157483
GR  - T32 CA009437/CA/NCI NIH HHS/United States
GR  - T32 RR007019/RR/NCRR NIH HHS/United States
GR  - T32CA 09437/CA/NCI NIH HHS/United States
GR  - P30-DK35816/DK/NIDDK NIH HHS/United States
GR  - T32 AG000057/AG/NIA NIH HHS/United States
GR  - P30 DK035816/DK/NIDDK NIH HHS/United States
GR  - T32 RR07019/RR/NCRR NIH HHS/United States
GR  - P01 AR021557/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Repressor Proteins)
RN  - 0 (retinoic acid receptor beta)
SB  - IM
MH  - Base Sequence
MH  - Breast Neoplasms/*metabolism
MH  - Cell Nucleus/metabolism
MH  - Cloning, Molecular
MH  - Cytoplasm/metabolism
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/genetics/metabolism
MH  - Open Reading Frames
MH  - Protein Biosynthesis
MH  - RNA, Messenger/metabolism
MH  - Receptors, Retinoic Acid/genetics/*metabolism
MH  - Repressor Proteins/genetics
MH  - Tumor Cells, Cultured
MH  - Up-Regulation
PMC - PMC17571
EDAT- 1999/07/21 00:00
MHDA- 1999/07/21 00:01
CRDT- 1999/07/21 00:00
PHST- 1999/07/21 00:00 [pubmed]
PHST- 1999/07/21 00:01 [medline]
PHST- 1999/07/21 00:00 [entrez]
AID - 10.1073/pnas.96.15.8651 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Jul 20;96(15):8651-6. doi:
      10.1073/pnas.96.15.8651.