PMID- 10411906
OWN - NLM
STAT- MEDLINE
DCOM- 19990823
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 15
DP  - 1999 Jul 20
TI  - Suppression of apoptosis signal-regulating kinase 1-induced cell death by 14-3-3 
      proteins.
PG  - 8511-5
AB  - Apoptosis signal-regulating kinase 1 (ASK1) is a pivotal component of a signaling
      pathway induced by many death stimuli, including tumor necrosis factor alpha,
      Fas, and the anticancer drugs cisplatin and paclitaxel. Here we report that ASK1 
      proapoptotic activity is antagonized by association with 14-3-3 proteins. We
      found that ASK1 specifically bound 14-3-3 proteins via a site involving Ser-967
      of ASK1. Interestingly, overexpression of 14-3-3 in HeLa cells blocked
      ASK1-induced apoptosis whereas disruption of the ASK1/14-3-3 interaction
      dramatically accelerated ASK1-induced cell death. Targeting of ASK1 by a
      14-3-3-mediated survival pathway may provide a novel mechanism for the
      suppression of apoptosis.
FAU - Zhang, L
AU  - Zhang L
AD  - Department of Pharmacology, Cell, and Developmental Biology, Emory University,
      Atlanta, GA 30322, USA.
FAU - Chen, J
AU  - Chen J
FAU - Fu, H
AU  - Fu H
LA  - eng
GR  - R01 GM053165/GM/NIGMS NIH HHS/United States
GR  - R29 GM053165/GM/NIGMS NIH HHS/United States
GR  - GM53165/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (14-3-3 Proteins)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinase 5)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinases)
RN  - EC 2.7.11.25 (MAP3K5 protein, human)
SB  - IM
MH  - 14-3-3 Proteins
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Cell Line
MH  - DNA Fragmentation/genetics
MH  - Gene Expression Regulation
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Luminescent Proteins
MH  - MAP Kinase Kinase Kinase 5
MH  - *MAP Kinase Kinase Kinases
MH  - Mutation
MH  - Phosphorylation
MH  - Protein Binding
MH  - Protein-Serine-Threonine Kinases/*genetics/metabolism
MH  - Proteins/*metabolism
MH  - Signal Transduction
MH  - Transfection
MH  - *Tyrosine 3-Monooxygenase
PMC - PMC17547
EDAT- 1999/07/21 00:00
MHDA- 1999/07/21 00:01
CRDT- 1999/07/21 00:00
PHST- 1999/07/21 00:00 [pubmed]
PHST- 1999/07/21 00:01 [medline]
PHST- 1999/07/21 00:00 [entrez]
AID - 10.1073/pnas.96.15.8511 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Jul 20;96(15):8511-5. doi:
      10.1073/pnas.96.15.8511.