PMID- 10411637
OWN - NLM
STAT- MEDLINE
DCOM- 19990806
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 262
IP  - 3
DP  - 1999 Jun
TI  - Complete nucleotide sequence, origin of isoform and functional characterization
      of the mouse hepsin gene.
PG  - 755-64
AB  - Hepsin, a type-II membrane-associated serine protease, has been implicated in
      cell growth and development as well as possible initiation of blood coagulation. 
      Here, we report on the complete nucleotide sequence, functional characterization 
      of key structural features and the promoter of the mouse hepsin gene. The gene
      has a size of approximately 17 kb, and is composed of 12, 13, or 14 exons
      depending on alternative intron splicings - one in the 5'-UTR and the other two
      in the second intron. The latter two, which occur in approximately half of the
      hepsin transcripts, generate a hepsin mRNA species with an extra exon, which is
      responsible for producing a hepsin isoform with a unique 20-residue sequence
      inserted in the cytoplasmic portion of hepsin. Most hepsin transcripts have the
      5'-UTR intron spliced, and its splicing can occur independently of the other
      alternative splicings. The transcriptional initiation site was determined to be
      636 bp upstream of the first ATG site in a cytidine-rich region. The 5'-flanking 
      region of hepsin up to nucleotide 274 showed a substantial promoter activity in
      HepG2 cells, with its expression activity sevenfold higher in the presence of the
      5'-UTR intron sequence in comparison to that without the intron sequence. The
      basal promoter region contains potential binding sites for several transcription 
      factors including SP1, AP2, C/EBP, LF-A1, and E box, which may be responsible for
      ubiquitous, but liver- and kidney-preferred tissue expression of the hepsin gene.
FAU - Kawamura, S
AU  - Kawamura S
AD  - Department of Human Genetics, University of Michigan Medical School, Ann Arbor
      48109-0618, USA.
FAU - Kurachi, S
AU  - Kurachi S
FAU - Deyashiki, Y
AU  - Deyashiki Y
FAU - Kurachi, K
AU  - Kurachi K
LA  - eng
GR  - 5960AR20557/AR/NIAMS NIH HHS/United States
GR  - HL38644/HL/NHLBI NIH HHS/United States
GR  - MO1RR00042/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Isoenzymes)
RN  - 0 (Membrane Proteins)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.- (hepsin)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Carcinoma, Hepatocellular
MH  - Humans
MH  - Isoenzymes/chemistry/genetics
MH  - Liver/chemistry
MH  - Membrane Proteins/chemistry/genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic/genetics
MH  - Serine Endopeptidases/*chemistry/*genetics
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1999/07/20 00:00
MHDA- 1999/07/20 00:01
CRDT- 1999/07/20 00:00
PHST- 1999/07/20 00:00 [pubmed]
PHST- 1999/07/20 00:01 [medline]
PHST- 1999/07/20 00:00 [entrez]
AID - ejb431 [pii]
AID - 10.1046/j.1432-1327.1999.00431.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Jun;262(3):755-64. doi: 10.1046/j.1432-1327.1999.00431.x.