PMID- 10410956
OWN - NLM
STAT- MEDLINE
DCOM- 19990914
LR  - 20190831
IS  - 1387-2273 (Print)
IS  - 1387-2273 (Linking)
VI  - 729
IP  - 1-2
DP  - 1999 Jun 11
TI  - Radiochemical assay for determination of dihydropyrimidinase activity using
      reversed-phase high-performance liquid chromatography.
PG  - 307-14
AB  - A radiochemical assay was developed to measure the activity of
      dihydropyrimidinase (DHP) in human liver homogenates. The method is based on the 
      separation of radiolabeled dihydrouracil from N-carbamyl-beta-alanine by HPLC
      with on-line detection of radioactivity combined with detection of 14CO2 by
      liquid scintillation counting. The assay was linear with time and protein
      concentration. The minimum amount of radiolabeled products which could be
      determined proved to be 12 pmol using a purified stock solution of
      [2-(14)C]-5,6-dihydrouracil. This highly sensitive assay is especially suitable
      to identify patients with a dihydropyrimidinase deficiency.
FAU - Van Kuilenburg, A B
AU  - Van Kuilenburg AB
AD  - Academic Medical Center, Laboratory Genetic Metabolic Diseases, Amsterdam, The
      Netherlands.
FAU - Van Lenthe, H
AU  - Van Lenthe H
FAU - Van Gennip, A H
AU  - Van Gennip AH
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr B Biomed Sci Appl
JT  - Journal of chromatography. B, Biomedical sciences and applications
JID - 9714109
RN  - 0 (Carbon Radioisotopes)
RN  - EC 3.5.- (Amidohydrolases)
RN  - EC 3.5.2.2 (dihydropyrimidinase)
SB  - IM
MH  - Amidohydrolases/*metabolism
MH  - Carbon Radioisotopes
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Kinetics
MH  - Liver/enzymology
MH  - Reproducibility of Results
MH  - Scintillation Counting
MH  - Sensitivity and Specificity
EDAT- 1999/07/20 00:00
MHDA- 1999/07/20 00:01
CRDT- 1999/07/20 00:00
PHST- 1999/07/20 00:00 [pubmed]
PHST- 1999/07/20 00:01 [medline]
PHST- 1999/07/20 00:00 [entrez]
AID - 10.1016/s0378-4347(99)00173-5 [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Sci Appl. 1999 Jun 11;729(1-2):307-14. doi:
      10.1016/s0378-4347(99)00173-5.