PMID- 10409713
OWN - NLM
STAT- MEDLINE
DCOM- 19990826
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 30
DP  - 1999 Jul 23
TI  - Fiz1, a novel zinc finger protein interacting with the receptor tyrosine kinase
      Flt3.
PG  - 21478-84
AB  - The receptor tyrosine kinase Flt3 has been shown to play a role in proliferation 
      and survival of hematopoietic progenitor cells as well as differentiation of
      early B lymphoid progenitors. However, the signaling events that control growth
      or differentiation are not completely understood. In order to identify new
      signaling molecules interacting with the cytoplasmic domain of Flt3, we performed
      a yeast two-hybrid screen. In addition to several SH2 domain-containing proteins,
      we have isolated a novel Flt3 interacting zinc finger protein (Fiz1) with 11
      C(2)H(2)-type zinc fingers. Fiz1 binds to the catalytic domain of Flt3 but not to
      the structurally related receptor tyrosine kinases Kit, Fms, and platelet-derived
      growth factor receptor. This association is independent of kinase activity. The
      interaction between Flt3 and Fiz1 detected in yeast was confirmed by in vitro and
      in vivo coprecipitation assays. Fiz1 mRNA is expressed in all murine cell lines
      and tissues tested. Anti-Fiz1 antibodies recognize a 60-kDa protein, which is
      localized in the nucleus as well as in the cytoplasm. Together, these results
      identified a novel class of interaction between a receptor tyrosine kinase and a 
      signaling molecule which is independent of the well established SH2
      domain/phosphotyrosine binding.
FAU - Wolf, I
AU  - Wolf I
AD  - Fred Hutchinson Cancer Research Center, Division of Basic Sciences, Seattle,
      Washington 98109-1024, USA. iwolf@fhcrc.org
FAU - Rohrschneider, L R
AU  - Rohrschneider LR
LA  - eng
SI  - GENBANK/AF126746
SI  - GENBANK/AF126747
GR  - CA40987/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Fiz1 protein, mouse)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Receptors, Cell Surface)
RN  - EC 2.7.10.1 (Flt3 protein, mouse)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (fms-Like Tyrosine Kinase 3)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carrier Proteins/*genetics/isolation & purification/*metabolism
MH  - Cloning, Molecular
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Proteins/*genetics/isolation & purification/*metabolism
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Receptor Protein-Tyrosine Kinases/*metabolism
MH  - Receptors, Cell Surface/metabolism
MH  - Sequence Alignment
MH  - Signal Transduction
MH  - Zinc Fingers
MH  - fms-Like Tyrosine Kinase 3
MH  - src Homology Domains
EDAT- 1999/07/20 00:00
MHDA- 1999/07/20 00:01
CRDT- 1999/07/20 00:00
PHST- 1999/07/20 00:00 [pubmed]
PHST- 1999/07/20 00:01 [medline]
PHST- 1999/07/20 00:00 [entrez]
AID - 10.1074/jbc.274.30.21478 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jul 23;274(30):21478-84. doi: 10.1074/jbc.274.30.21478.