PMID- 10409705
OWN - NLM
STAT- MEDLINE
DCOM- 19990826
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 30
DP  - 1999 Jul 23
TI  - Selective association of G protein beta(4) with gamma(5) and gamma(12) subunits
      in bovine tissues.
PG  - 21425-9
AB  - The beta and gamma subunits of G proteins are tightly bound under physiological
      conditions, and so far, seven beta and 11 gamma subunit isoforms have been found.
      The relative abilities of the beta and gamma subunits to associate with each
      other have been studied using transfected cell assays, in vitro translation and
      the yeast two-hybrid system, but have not been fully characterized in various
      tissues. In the present study, we demonstrated the selectivity of association of 
      the beta with gamma isoforms in bovine tissues. Immunoprecipitation of betagamma 
      complexes from tissue extracts with antibodies against various gamma subunits and
      subsequent analyses revealed that beta(4) associated with the gamma subunits with
      the following rank order of selectivity: gamma(5) > gamma(12) > gamma(2) >
      gamma(3), while beta(2) bound to gamma(2), gamma(3), and gamma(12) more
      selectively than to gamma(5). By contrast, beta(1) associated with all gamma
      subunits without significant selectivity. Analyses of purified betagamma
      complexes containing various gamma isoforms revealed beta subunit compositions
      similar to those found in the immunoprecipitates. Particular combinations of beta
      and gamma subunit isoforms may contribute to maintaining efficient and specific
      signal transduction mediated by G proteins.
FAU - Asano, T
AU  - Asano T
AD  - Department of Biochemistry, Institute for Developmental Research, Aichi Human
      Service Center, Kasugai, Aichi 480-0392, Japan. toasano@inst-hsc.pref.aichi.jp
FAU - Morishita, R
AU  - Morishita R
FAU - Ueda, H
AU  - Ueda H
FAU - Kato, K
AU  - Kato K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Protein Isoforms)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cattle
MH  - GTP-Binding Proteins/*chemistry/metabolism
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Protein Conformation
MH  - Protein Isoforms/*chemistry/metabolism
EDAT- 1999/07/20 00:00
MHDA- 1999/07/20 00:01
CRDT- 1999/07/20 00:00
PHST- 1999/07/20 00:00 [pubmed]
PHST- 1999/07/20 00:01 [medline]
PHST- 1999/07/20 00:00 [entrez]
AID - 10.1074/jbc.274.30.21425 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jul 23;274(30):21425-9. doi: 10.1074/jbc.274.30.21425.