PMID- 10409679
OWN - NLM
STAT- MEDLINE
DCOM- 19990826
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 30
DP  - 1999 Jul 23
TI  - Human mitochondrial carbonic anhydrase VB. cDNA cloning, mRNA expression,
      subcellular localization, and mapping to chromosome x.
PG  - 21228-33
AB  - A cDNA clone for a novel carbonic anhydrase (CA) isozyme was isolated from human 
      pancreas and salivary glands. The cDNA sequence of 1182 base pairs encoded a
      317-amino acid protein with a predicted mass of 36.4 kDa. The highest similarity 
      of this cDNA and the deduced amino acid sequence is to human CA V (mitochondrial 
      CA), hereafter referred to as CA VA. Recombinant protein expressed in COS-7 cells
      transfected with this cDNA clone was enriched in a mitochondrial fraction.
      Confocal fluorescence microscopy showed cytoplasmic granular signals in COS-7
      cells expressing a fusion protein of the novel CA and green fluorescent protein. 
      Several lines of evidence suggest that the cDNA clone presented herein encodes a 
      novel human mitochondrial CA isozyme, designated CA VB. CA VB has a hydrophobic
      N-terminal mitochondrial signal sequence (33 amino acid residues). Western blot
      analysis showed a 36-kDa protein precursor and a 32-kDa mature protein for CA VB.
      Similar to CA VA, CA VB is a "low activity" enzyme with a sensitivity to
      acetazolamide. The CA VB gene is located on Xp22.1. Northern blot analysis in
      normal human tissues demonstrated expression of a 1.3-kilobase transcript in
      heart and skeletal muscle, and reverse transcription-polymerase chain reaction
      analysis showed expression of CA VB in pancreas, kidney, salivary glands, and
      spinal cord but not in liver. CA VA mRNA expression was observed only in liver.
      These findings indicate these are two genetically distinct isoforms of human CA
      V, designated CA VA and CA VB, which have different patterns of tissue-specific
      distribution, suggest different physiological roles for the two mitochondrial
      isozymes.
FAU - Fujikawa-Adachi, K
AU  - Fujikawa-Adachi K
AD  - First Department of Internal Medicine, Kochi Medical School, Nankoku, Kochi
      783-8505, Japan.
FAU - Nishimori, I
AU  - Nishimori I
FAU - Taguchi, T
AU  - Taguchi T
FAU - Onishi, S
AU  - Onishi S
LA  - eng
SI  - GENBANK/AB021660
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Carbonic Anhydrases/*genetics/metabolism
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA, Complementary/analysis/genetics
MH  - Humans
MH  - Mitochondria/*enzymology
MH  - Molecular Sequence Data
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Sequence Alignment
MH  - *X Chromosome
EDAT- 1999/07/20 00:00
MHDA- 1999/07/20 00:01
CRDT- 1999/07/20 00:00
PHST- 1999/07/20 00:00 [pubmed]
PHST- 1999/07/20 00:01 [medline]
PHST- 1999/07/20 00:00 [entrez]
AID - 10.1074/jbc.274.30.21228 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jul 23;274(30):21228-33. doi: 10.1074/jbc.274.30.21228.