PMID- 10409671
OWN - NLM
STAT- MEDLINE
DCOM- 19990826
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 30
DP  - 1999 Jul 23
TI  - FYN-T-FYB-SLP-76 interactions define a T-cell receptor zeta/CD3-mediated tyrosine
      phosphorylation pathway that up-regulates interleukin 2 transcription in T-cells.
PG  - 21170-9
AB  - Protein-tyrosine kinases p56(Lck), SYK, and ZAP-70 and downstream adaptors LAT
      and SLP-76 have been implicated as essential components in T-cell activation.
      Another lymphoid-specific adaptor FYB/SLAP has also been identified as a
      predominant binding partner of SLP-76 and the Src kinase FYN-T, although its role
      in the activation process has been unclear. In this study, we demonstrate that
      FYN-T selectively phosphorylates FYB providing a template for the recruitment of 
      FYN-T and SLP-76 SH2 domain binding. This interaction is unusual in its distinct 
      cytoplasmic localization and its long term stable kinetics of phosphorylation.
      Furthermore, we demonstrate for the first time that the co-expression of all
      three components of the FYN-T-FYB-SLP-76 matrix can synergistically up-regulate
      T-cell receptor-driven interleukin 2 transcription activity. These findings
      document the existence of a T-cell receptor-regulated FYN-T-FYB pathway that
      interfaces with the adaptor SLP-76 and up-regulates lymphokine production in
      T-cells.
FAU - Raab, M
AU  - Raab M
AD  - Division of Tumor Immunology, Department of Cancer Immunology and AIDS, Dana
      Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115,
      USA.
FAU - Kang, H
AU  - Kang H
FAU - da Silva, A
AU  - da Silva A
FAU - Zhu, X
AU  - Zhu X
FAU - Rudd, C E
AU  - Rudd CE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (CD3 Complex)
RN  - 0 (Interleukin-2)
RN  - 0 (Phosphoproteins)
RN  - 0 (SLP-76 signal Transducing adaptor proteins)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - CD3 Complex/*metabolism
MH  - Enzyme Activation
MH  - Humans
MH  - Interleukin-2/*biosynthesis/genetics/immunology
MH  - Jurkat Cells
MH  - Lymphocyte Activation
MH  - Mice
MH  - Phosphoproteins/metabolism
MH  - Phosphorylation
MH  - *Protein-Tyrosine Kinases/*metabolism
MH  - Signal Transduction/*immunology
MH  - T-Lymphocytes/immunology/*metabolism
MH  - Transcription, Genetic
MH  - Tyrosine/metabolism
MH  - Up-Regulation
EDAT- 1999/07/20 00:00
MHDA- 1999/07/20 00:01
CRDT- 1999/07/20 00:00
PHST- 1999/07/20 00:00 [pubmed]
PHST- 1999/07/20 00:01 [medline]
PHST- 1999/07/20 00:00 [entrez]
AID - 10.1074/jbc.274.30.21170 [doi]
AID - S0021-9258(19)72560-5 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Jul 23;274(30):21170-9. doi: 10.1074/jbc.274.30.21170.