PMID- 10409670
OWN - NLM
STAT- MEDLINE
DCOM- 19990826
LR  - 20191210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 30
DP  - 1999 Jul 23
TI  - Characterization of the murine mafF gene.
PG  - 21162-9
AB  - Small Maf proteins are obligatory heterodimeric partner molecules of mammalian
      Cap'n'Collar proteins that together control a wide variety of eukaryotic genes.
      Although both MafK and MafG are expressed in overlapping but distinct tissue
      distribution patterns during embryonic development, the physiological
      consequences of loss-of-function mutations in either gene are modest. This
      suggested that compensation by the third small Maf protein, MafF, might be a
      major reason for such mild phenotypes and that further analysis of MafF might
      therefore provide important insights for understanding small Maf regulatory
      function(s). We therefore cloned, mapped, transcriptionally and developmentally
      characterized, and finally disrupted the mafF gene. We show that murine mafF is
      transcriptionally regulated by three different promoters and is most abundantly
      expressed in the lung. The lacZ gene inserted into the mafF locus revealed
      prominent expression sites in the gut, lung, liver, outflow tract of the heart,
      cartilage, bone membrane, and skin but not in hematopoietic cells at any
      developmental stage. Homozygous mafF null mutant mice were born in a normal
      Mendelian ratio and displayed no obvious functional deficiencies, indicating that
      MafF activity may be dispensable even in tissues where the expression of other
      small Maf proteins is quite low.
FAU - Onodera, K
AU  - Onodera K
AD  - Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern
      University, Evanston Illinois 60208-3500, USA.
FAU - Shavit, J A
AU  - Shavit JA
FAU - Motohashi, H
AU  - Motohashi H
FAU - Katsuoka, F
AU  - Katsuoka F
FAU - Akasaka, J E
AU  - Akasaka JE
FAU - Engel, J D
AU  - Engel JD
FAU - Yamamoto, M
AU  - Yamamoto M
LA  - eng
SI  - GENBANK/AB009693
SI  - GENBANK/AB009694
GR  - CA60553/CA/NCI NIH HHS/United States
GR  - CA80088/CA/NCI NIH HHS/United States
GR  - GM08152/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MafF Transcription Factor)
RN  - 0 (Maff protein, mouse)
RN  - 0 (Nuclear Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins
MH  - *Gene Expression Regulation
MH  - *Genome
MH  - Leucine Zippers/genetics
MH  - MafF Transcription Factor
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Nuclear Proteins/*genetics
MH  - Sequence Alignment
EDAT- 1999/07/20 00:00
MHDA- 1999/07/20 00:01
CRDT- 1999/07/20 00:00
PHST- 1999/07/20 00:00 [pubmed]
PHST- 1999/07/20 00:01 [medline]
PHST- 1999/07/20 00:00 [entrez]
AID - 10.1074/jbc.274.30.21162 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jul 23;274(30):21162-9. doi: 10.1074/jbc.274.30.21162.