PMID- 10409623
OWN - NLM
STAT- MEDLINE
DCOM- 19990826
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 30
DP  - 1999 Jul 23
TI  - Molecular cloning of transferrin receptor 2. A new member of the transferrin
      receptor-like family.
PG  - 20826-32
AB  - Transferrin receptor (TfR) plays a major role in cellular iron uptake through
      binding and internalizing a carrier protein transferrin (Tf). We have cloned,
      sequenced, and mapped a human gene homologous to TfR, termed TfR2. Two
      transcripts were expressed from this gene: alpha (approximately 2.9 kilobase
      pairs), and beta (approximately 2.5 kilobase pairs). The predicted amino acid
      sequence revealed that the TfR2-alpha protein was a type II membrane protein and 
      shared a 45% identity and 66% similarity in its extracellular domain with TfR.
      The TfR2-beta protein lacked the amino-terminal portion of the TfR2-alpha protein
      including the putative transmembrane domain. Northern blot analysis showed that
      the alpha transcript was predominantly expressed in the liver. In addition, high 
      expression occurred in K562, an erythromegakaryocytic cell line. To analyze the
      function of TfR2, Chinese hamster ovary TfR-deficient cells (CHO-TRVb cells) were
      stably transfected with FLAG-tagged TfR2-alpha. These cells showed an increase in
      biotinylated Tf binding to the cell surface, which was competed by nonlabeled Tf,
      but not by lactoferrin. Also, these cells had a marked increase in Tf-bound
      (55)Fe uptake. Taken together, TfR2-alpha may be a second transferrin receptor
      that can mediate cellular iron transport.
FAU - Kawabata, H
AU  - Kawabata H
AD  - Cedars-Sinai Medical Center, Department of Medicine, Division of
      Hematology/Oncology, Burns and Allen Research Institute, University of California
      Los Angeles School of Medicine, Los Angeles, California 90048, USA.
      hkawabat@ucla.edu
FAU - Yang, R
AU  - Yang R
FAU - Hirama, T
AU  - Hirama T
FAU - Vuong, P T
AU  - Vuong PT
FAU - Kawano, S
AU  - Kawano S
FAU - Gombart, A F
AU  - Gombart AF
FAU - Koeffler, H P
AU  - Koeffler HP
LA  - eng
SI  - GENBANK/AF067864
GR  - CA26038-20/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Receptors, Transferrin)
RN  - 0 (TFR2 protein, human)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - Humans
MH  - Iron/*metabolism
MH  - K562 Cells
MH  - Molecular Sequence Data
MH  - Receptors, Transferrin/*genetics/metabolism
MH  - Sequence Alignment
MH  - Sequence Analysis
MH  - Signal Transduction
EDAT- 1999/07/20 00:00
MHDA- 1999/07/20 00:01
CRDT- 1999/07/20 00:00
PHST- 1999/07/20 00:00 [pubmed]
PHST- 1999/07/20 00:01 [medline]
PHST- 1999/07/20 00:00 [entrez]
AID - 10.1074/jbc.274.30.20826 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jul 23;274(30):20826-32. doi: 10.1074/jbc.274.30.20826.