PMID- 10408446
OWN - NLM
STAT- MEDLINE
DCOM- 19990723
LR  - 20190617
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 400
IP  - 6740
DP  - 1999 Jul 8
TI  - Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic
      spindle formation.
PG  - 178-81
AB  - Chromosomes are segregated by two antiparallel arrays of microtubules arranged to
      form the spindle apparatus. During cell division, the nucleation of cytosolic
      microtubules is prevented and spindle microtubules nucleate from centrosomes (in 
      mitotic animal cells) or around chromosomes (in plants and some meiotic cells).
      The molecular mechanism by which chromosomes induce local microtubule nucleation 
      in the absence of centrosomes is unknown, but it can be studied by adding
      chromatin beads to Xenopus egg extracts. The beads nucleate microtubules that
      eventually reorganize into a bipolar spindle. RCC1, the
      guanine-nucleotide-exchange factor for the GTPase protein Ran, is a component of 
      chromatin. Using the chromatin bead assay, we show here that the activity of
      chromosome-associated RCC1 protein is required for spindle formation. Ran itself,
      when in the GTP-bound state (Ran-GTP), induces microtubule nucleation and
      spindle-like structures in M-phase extract. We propose that RCC1 generates a high
      local concentration of Ran-GTP around chromatin which in turn induces the local
      nucleation of microtubules.
FAU - Carazo-Salas, R E
AU  - Carazo-Salas RE
AD  - EMBL, Heidelberg, Germany.
FAU - Guarguaglini, G
AU  - Guarguaglini G
FAU - Gruss, O J
AU  - Gruss OJ
FAU - Segref, A
AU  - Segref A
FAU - Karsenti, E
AU  - Karsenti E
FAU - Mattaj, I W
AU  - Mattaj IW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cell Extracts)
RN  - 0 (Chromatin)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RCC1 protein, Xenopus)
RN  - 0 (RCC1 protein, human)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Xenopus Proteins)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.5.2 (ran GTP-Binding Protein)
SB  - IM
MH  - Animals
MH  - *Cell Cycle Proteins
MH  - Cell Extracts
MH  - Chromatin/*physiology
MH  - DNA-Binding Proteins/*physiology
MH  - *Guanine Nucleotide Exchange Factors
MH  - Guanosine Triphosphate/metabolism/*physiology
MH  - Humans
MH  - In Vitro Techniques
MH  - Microtubules/physiology
MH  - Mitosis/*physiology
MH  - Models, Biological
MH  - Mutation
MH  - Nuclear Proteins/genetics/metabolism/*physiology
MH  - Recombinant Proteins
MH  - Schizosaccharomyces
MH  - Signal Transduction
MH  - Spindle Apparatus/*physiology
MH  - Xenopus Proteins
MH  - Xenopus laevis
MH  - ran GTP-Binding Protein
EDAT- 1999/07/17 10:00
MHDA- 2001/03/23 10:01
CRDT- 1999/07/17 10:00
PHST- 1999/07/17 10:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/07/17 10:00 [entrez]
AID - 10.1038/22133 [doi]
PST - ppublish
SO  - Nature. 1999 Jul 8;400(6740):178-81. doi: 10.1038/22133.